Application of the NanoLC-MS/MS Technique for Protein Analysis of Biofilm on Surface of Mandibular Fixation with X-Ray Detection of Metallic Ions Relocation to the Osseous Tissue

Abstract:

This work presents application of the methods of combined analytical techniques: high performance capillary chromatography and mass spectrometry with a nanoelectrospray ion source (nanoESI) for biochemical analysis of biological films. The layers of biofilm (identification of surface proteins) adsorbed on surfaces of metallic plate fixation and metallic screws surfaces used during treatment of the mandibular fracture were investigated. The obtained results make it possible to determine the protein composition of films found on the plates and screws used for fixation of the mandible. Second part of the work involves microscopic observations of specially prepared parts of tissues contacting directly with time-part implant. We observed changes in the osseous tissues and relocations of the metal ions from the fixations using a Hitachi S-3000N scanning microscope, equipped with an NSS type (Noran System Six) X-ray spectrometer and a freezing table for biological sections. Obtained results indicate the penetration of metal ions from the time-part implant surface into the osseous tissue.

• Periodical: Solid State Phenomena (Volume 199)
• Pages: 531-537
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.199.531
• Online since: March 2013
• Authors: Joanna Mystkowska, Piotr Suder, Jerzy Silberring, Anna Drabik, Jarosław Sidun, Stanisława Zyta Grabowska, Jan Borys, Jan Ryszard Dąbrowski
• Keywords: Biofilm, LC-MS/MS Technique, Mandibular Fixation

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