Agarose-Chitosan Based Hydrogel Waveguide Matrix: Comparison Synthesis and Performance for Optical Leaky Waveguide (OLW) Biosensor

Abstract:

In this work, a hydrogel-based optical leaky waveguide (OLW) biosensor was developed to detect biomolecules using changes in refractive index (Dn). Hydrogels formed from chitosan and agarose were synthesised to be used as a waveguiding thin film in OLW biosensor. The concentration and thickness of the hydrogels defined by the spin coater speeds and time were optimised to produce a device chip with a single waveguide moded operation of OLW biosensor. The cladding layer was fabricated using 9.5nm titanium coated glass substrate for metal-clad (MCLW) and addition of dye particles (reactive blue 4) for dye-clad (DCLW) format. For the performance of the fabricated device for detection of Dn of a glycerol solution, the highest sensitivity of detection was obtained from MCLW chip made of 2% w/v of chitosan-agarose hydrogel spun at 6000 rpm with LOD: 4.28 x 10^–6 RIU, while for DCLW format, the LOD measured at 7.46 x 10^–6 RIU. The performance of the device to monitor the protein (bovine serum albumin; BSA and anti-BSA) interaction, the binding affinity in respect of change in peak angle (DӨ°) of chitosan matrix to the protein was measured at DӨ°~(6.2 ± 0.5°)x10^–6.

• Periodical: Solid State Phenomena (Volume 301)
• Pages: 87-96
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.301.87
• Online since: March 2020
• Authors: Siti Rabizah Makhsin*, Peter Gardner, Nicholas J. Goddard, Patricia J. Scully
• Keywords: Agarose Chitosan, Hydrogel, Leaky Waveguide, Optical Biosensor, Protein Interaction

References

[1] J. Tavakoli, Y. Tang, Hydrogel based sensors for biomedical applications: an updated review, Polymers, 9 (2017) 364.

DOI: 10.3390/polym9080364

[2] R.L. Rich, G.A. Papalia, P.J. Flynn, J. Furneisen, J. Quinn, J.S. Klein, et al., A global benchmark study using affinity-based biosensors, Anal. Biochem., 386.2 (2009) 194-216.

[3] M. Zourob, S. Mohr, B.J.T. Brown, P.R. Fielden, M.B. McDonnell, N.J. Goddard, An integrated metal clad leaky waveguide sensor for detection of bacteria, Anal. Chem., 77 (2005) 232-42.

DOI: 10.1021/ac049627g

[4] R. Gupta, B. Bastani, N.J. Goddard, B. Grieve, Absorption spectroscopy in microfluidic flow cells using a metal clad leaky waveguide device with a porous gel waveguide layer, Analyst, 138 (2013) 307-14.

DOI: 10.1039/c2an35898k

[5] M. Zourob, N.J. Goddard, Metal clad leaky waveguides for chemical and biosensing applications, Biosens Bioelectron, 20 (2005) 1718-27.

DOI: 10.1016/j.bios.2004.06.031

[6] M. Zourob, S. Mohr, B.J.T. Brown, P.R. Fielden, M. McDonnell, N.J. Goddard, The development of a metal clad leaky waveguide sensor for the detection of particles, Sensor Actuat B-Chem, 90 (2003) 296-307.

DOI: 10.1016/s0925-4005(03)00052-2

[7] Y. Miao, K. Zhang, Y. Yuam, B. Liu, H. Zhang, Y. Liu, et al., Agarose gel-coated LPG based on two sensing mechanisms for relative humidity measurement, Appl. Opt., 52 (2013) 90-5.

DOI: 10.1364/ao.52.000090

[8] Z. Cao, R.J. Gilbert, W. He, Simple Agarose − Chitosan gel composite system for enhanced neuronal growth in three dimensions, Biomacromolecules, 10 (2009) 2954-9.

DOI: 10.1021/bm900670n

[9] F.J. Arregui, Z. Ciaurriz, M. Oneca, I.R. Matıas, An experimental study about hydrogels for the fabrication of optical fiber humidity sensors, Sensor Actuat B-Chem, 96 (2003) 165-72.

DOI: 10.1016/s0925-4005(03)00520-3

[10] K.J. Lee, D. Wawro, P.S. Priambodo, R. Magnusson, Agarose-gel based guided-mode resonance humidity sensor, IEEE Sens. J., 7 (2007) 409-14.

DOI: 10.1109/jsen.2006.890129

[11] A. Jain, A.H. Yang, D. Erickson, Gel-based optical waveguides with live cell encapsulation and integrated microfluidics, Opt. Lett., 37 (2012) 1472-4.

DOI: 10.1364/ol.37.001472

[12] A.K. Agarwal, S. Singla, A. Kumar, Hypokalemic Paralysis: Emergency Room Management, in: A.K. Agarwal (Ed), Medicine Update, Noida, Association of Physicians of India, 2009, p.42–9.

[13] S. Ladet, L. David, A. Domard, Multi-membrane hydrogels, Nature, 452 (2008) 76-79.

DOI: 10.1038/nature06619

[14] M. Dash, F. Chiellini, R.M. Ottenbrite, E. Chiellini, Chitosan: A versatile semi-synthetic polymer in biomedical applications, Prog. Polym. Sci., 36 (2011) 981-1014.

DOI: 10.1016/j.progpolymsci.2011.02.001

[15] H. Yi, L.Q. Wu, W.E. Bentley, R. Ghodssi, G.W. Rubloff, J.N. Culver, et al., Biofabrication with Chitosan, Biomacromolecules, 6 (2005) 2881-94.

DOI: 10.1021/bm050410l

[16] S.R. Makhsin, P. Gardner, N.J. Goddard, P.J. Scully, Surface modification of titanium-coated glass substrate embedded acrylate-based hydrogel film for optical metal clad leaky waveguide (MCLW) biosensors, 2017 IEEE SENSORS, (2017) 1-3.

DOI: 10.1109/icsens.2017.8234289

[17] S.K. Shukla, A.K. Mishra, O.A. Arotiba, B.B. Mamba, Chitosan-based nanomaterials: A state-of-the-art review, Int. J. Biol. Macromol., 59(2013) 46-58.

DOI: 10.1016/j.ijbiomac.2013.04.043

[18] G.T. Hermanson, PEGylation and Synthetic Polymer Modification, Bioconjugate techniques, Academic press, 2013, pp.787-838.

DOI: 10.1016/b978-0-12-382239-0.00018-2