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Thermostability Evolution of the Xylanase Yanling Yang1,a, Wei Zhang1,b, Huixiang Lian1,c, Jiadong Huang2,d, Heng Li1,e, and Shihua Wang1,f,* 1The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 School of Biological & Medical, University of Jinan, Jinan 250022, China ayylwyh@126.com, bzhwei513@yahoo.com.cn, c-273k1983@163.com, dyichenhuang1127@hotmail.com, eleehome1985@163.com, f, *Corresponding author: wshyyl@sina.com Keywords: Xylanases, Clone, Expression, Directed evolution, Thermostability Abstract: Xylanase (E.C 3.2.1.8) is a widespread group of enzyme which can catalyze the endohydrolysis of 1, 4-β-D-xylosidic linkages in xylan.
Xylanases play important roles in xylan hydrolyzation, so they are widely used in biopulping wood, bleaching pulp, and bioconversion of lignocellulose material to fermentative products [2].
Using these as the starting material for further iterations of mutagenesis and selection can yield proteins with significantly enhanced or novel functions.
Materials and Methods Microorganisms, Culture Conditions and Chemicals.
Leisola: Protein engineering.

The Study of Multiplex PCR Conditions for Detection of three Food-borne Bacterial Pathogens Ai Li1, a，Guo Xing Yang2,b ，Wei Zhang3,c 1 Environmental and Chemical Engineering of Tangshan College, China 2 Handan Municipal Center for Disease Prevention and Control, China 3 College of Food Science and Technology of Agricultural University of Hebei , China aaiai3252891@163.com, b yangguoxing888@126.com, czhangwei631126@yahoo.com.cn Key words: Multiplex PCR ; Food-borne Pathogens ; Detection; FTA filter Abstract.
Materials and Methods Experimental Materials Bacterial Species.

Comparative study of microstructural and corrosion characteristics of excimer laser-melted AA2124-T4 and AA6061-T4 alloys Daishu Qian*, Zhu Liu Corrosion and Protection Centre, School of Materials, the University of Manchester, Manchester, M13 9PL, UK daishu.qian@postgrad.manchester.ac.uk Key Words: Aluminium alloy; Laser surface melting; Corrosion Abstract.
The porosity observed in the melted layer could be resulted from the release of hydrogen stored in the materials or the trapped gases in existing cracks on re-irradiation [8].
Stampanoni, Corrosion protection of AA2024-T351 friction stir welds by laser surface melting with Excimer laser, Corrosion Engineering, Science and Technology 47 (2012) 188-202.

Summarizing, highly dispersed titanium oxide in mesoporous silica materials leads to relatively high yield in CH4 and CH3OH.
Industrial & Engineering Chemistry Research, 45 (8) (2006) 2558-2568
Nature Materials 5(8) (2006) 665-670
Selloni, One step towards bridging the materials gap: surface studies of TiO2 anatase.
Physical Review B: Condensed Matter and Materials Physics, 28(6) (1983) 3427-38

Due to the difference between solid materials and pure water on concentration, density, hardness, particle size and viscosity, etc.
ASME Journal of Basic Engineering, Ser.
Fluid Engineering, 1991, 3(5), p 17-19
Journal of Engineering Thermophysics, 2001, 22(4), p 450-452
Journal of Engineering Thermophysics, 2006, 27(3), p 420-422

Journal of hydraulic engineering, 2003, 34(3): 55-62.
Journal of hydraulic engineering, 2011, 42(2): 152-159.
Chinese Journal of Rock Mechanics and Engineering, 2011, 30(5): 910-916.
China Civil Engineering Journal, 2006, 39(7): 81–87.
Chinese Journal of Rock Mechanics and Engineering, 2010, 29(3): 433-441.

Effects of sewage sludge application on contents of heavy metals in the soil and growth of maize Liang Dai1,a *, Yamei Gao1,b, Junping Zhang1,c (1.School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, P.
Material and Methods The sewage sludge was obtained from the An-ning Plant of Wastewater Treatment of Lanzhou City.
The effect of heavy metals in the soil on plant root system must be a key consideration at sludge application.
Gao, et al, Key problems in municipal sludge composting and its industrialization process, China Water & Waste Water. 25(2009)104-108(In Chinese)
Studies on land application of sewage sludge and its limiting factors, Journal of Hazardous Materials. 160 (2008) 554-558

Experimental Analysis for the Low-Temperature Growth of Poly-Si Films by Using Double Excimer Laser Long-Sun Chao 1, a and Yu-Ru Chen 2,b 1,2 Department of Engineering Science, National Cheng Kung University; No.1, University Road, Tainan City, 701, Taiwan, R.O.C.
Accordingly, how to enhance the absorbed energy of the silicon film and to avoid the substrate deformation is the key point of the fabricated process.
Consequently, how to increase the lateral growth is another key point of the relating studies.
Those methods mentioned above mainly apply the views of the material, optics and partial heat transfer to produce big grains.
[9] Yu-Ru Chen and Long-Sun Chao, Materials Science Forum, Vols.505-507, pp. 277-282 , 2006

New Materials Research Institute of Shandong Academy of Sciences, Jinan, China. 2.
School of Environmental Science & Engineering of Shandong University, Jinan, China.
aemail:zhuyingee@163.com, b email:25411102@qq.com, c email: hanzhe@sdu.edu.cn, demail: fengsp@sdu.edu.cn Key words: PAHs, PCBs, OCPs, degradation, sludge, landfill Abstract: In order to know about the variation of PAHs, PCBs and OCPs of sludge in landfill, discover the factors influencing the contents of PAHs, PCBs, OCPs and provide the scientific basis for the agriculture reuse of aged-sludge, the variation of PAHs, PCBs, OCPs’ contents and influencing factors in sludge landfill were studied in this paper.
Material and Methods Sampling and Storage.
Therefore, the key work of doing source apportionment of pollutants in sludge lies in ascertaining the main source of pollutants in wastewater.

Mortar and brick are common materials that used in building up a structure since several years ago.
Now, bricks masonry still widely used material along with other material.
For proportion specification, mortar is defined by volume ratio of the materials which are used to constitute mortar.
It had been studied of mortar joints by using two mortars consisting of two different quality materials along the bond length.
Whereas lightweight brick which has low compressive strength due to low-density materials that could contain up to 50 per cent of restricted air, subsequently, this may have an effect on the results.