Thermostability Evolution of the Xylanase

Abstract:

Xylanase (E.C 3.2.1.8) is a widespread group of enzyme which can catalyze the endohydrolysis of 1, 4-β-D-xylosidic linkages in xylan. The thermostable xylanase has attracted widely attention because of its application in industrial processes. In this study, the cDNA of xylanase gene was obtained from Aspergillus niger C3486 by RT-PCR. The xylanase DNA contains a 50 bp intron through the comparison between the DNA and cDNA sequence. The thermostability of this xylanase was significantly improved by directed evolution.

• Periodical: Advanced Materials Research (Volumes 236-238)
• Pages: 974-980
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.236-238.974
• Online since: May 2011
• Authors: Yan Ling Yang, Wei Zhang, Hui Xiang Lian, Jia Dong Huang, Heng Li, Shi Hua Wang
• Keywords: Clone, Directed Evolution, Expression, Thermostability, Xylanases

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