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Third, because valves will be in direct contact with PCR solution, the valve material should not inhibit PCR.
Quake, “Monolithic microfabricated valves and pumps by multilayer soft lithography,” Science, 2000, pp. 113-116, doi: 10.1126/science.288. 5463.113
Backhouse, “An adaptable microvalving system for on-chip polymerase chain reactions,” Journal of Immunological Methods, vol. 305, 2005, pp. 48–58,doi: 10. 1016/j.jim.2005.07.009

(6) ρ: Density of screw material [kg/m3]; l : Length of screw [m]; d : Diameter of screw [m]; (c): Moment of inertia of coupling: CJ So, Moment of inertia of system load: MB C J J J J = + +
[2] Liu Yueqin, Yin Shangxin: Journal of South China University of Technology, Vol. 28 (2000), p.105-109.
[4] Jiang Niandong, Digital Servo Control System and its Emulation for Absorbing Wave Maker, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, 1992.
[8] Li Hong: Practical Handbook of Machine Design, Liaoning Science and Technology Press, 1999.

Shock Vibration and Structural Trauma of a Torpedoed Ship M G Wood 1 D I Smith 2 M R Hayns 3 1 Combs, High Peak, UK 2 School of Engineering and Applied Science, Aston University, Birmingham, UK 3 West Runton, Norfolk, UK Keywords: WW1, Sinking, Steamship, U-boat, Vibration, Structural Failure Abstract On an afternoon in May of 1915, whilst sailing to Liverpool from New York, the Cunard Liner Lusitania was torpedoed by a German U-boat and she sank costing the lives of nearly 1200 passengers and crew.
Our thanks also to the staff of the Royal Navy Submarine Museum in Gosport for supplying U-Boat torpedo information, the National Maritime Museum in Liverpool for allowing access to related Cunard Collection archive material, and Gillard Welch Ltd. of London for permission to reproduce diagrams from the original Engineering journal on the S.S.
References: [1] Wood MG et al; Lusitania: Reviewing the Evidence; Science & Justice, Volume 42, 3, July/September 2002; pp 173 - 188 [2] Preston D; Wilful Murder: the sinking of the Lusitania; Doubleday Books, London, 2002

Acknowledgments The authors acknowledge the financial support by the National Natural Science Foundation of China (Grant no. 51179082), and by the Natural Science Foundation of Shandong Province (ZR2010EL035).
Journal of Intelligent Material Systems and Structures (1994), Vol.5, pp.585-589

Seismic Analysis of Separated Platform Subway Station Structure Yao Zhou 1, a, Zhiwei Wang 2 , Yuanfeng Wang 3 1School of Transportation Science and Engineering, Beihang University, Beijing 100191 China 2 Hebei Construction Group Corporation Limited., Hebei 050051, China 3 School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China azhouyao@buaa.edu.cn Keywords: Seismic Analysis ; Subway Station Structure; Separated Platform Abstract.
Acknowledgements This research is financial supported by the Fundamental Research Funds for the Central Universities under the grant number YWF-10-02-061 and the National Natural Science Foundation of China under the grant number 51008011.
Journal of Disaster Prevention and Mitigation Engineering, 26(2006) , p.268–273.
Proceedings of International Conference on Health Monitoring of Structure, Material and Environment, Nanjing, China(2007) , p.342-346

Furthermore, the model will discover and solve fault equipments in time, and makes the contradictions and conflicts in the maintenance process clearly to improve the management efficiency and the personnel's reaction rate, so the supply of human, material and financial resources will be guaranteed.
Acknowledgements The research was supported by the foundation under the grant No.71071043 from National Natural Science Foundation of China References [1] K.
Tan: Science & Technology Progress and Policy, Vol. 26 (2009) No. 21, p. 47.
Calis: Journal of Construction Engineering and Management-ASCE, Vol. 138 (2012) No. 3, p. 431

Research on the Differences of the Pore Characteristics with Different Destroyed-Type Coals Ling ling Qi1,a, Zhao feng Wang2,b, Hong min Yang3,c 1,2,3School of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo 454003, China; 1,2,3Key Laboratory of Gas Geology and Gas Control of Henan Province——State Key Laboratory Breeding Base with province-ministry co-constuct, Jiaozuo 454003, China aqll5407@163.com, bwzf3988@163.com, cyhmfs@163.com Key words: coal, pores, destroyed-type, coal and gas outburst Abstract: In order to investigate the pore characteristics of the different destroyed-types of coals and its effects of coal and gas outburst, this article studyed the low temperature nitrogen adsorption curve, specific surface area, pore volume and its distribution with the method of low-temperature nitrogen adsorption, also studed their changes with the different destroyed-type of coals, and the influences of pore shape and distribution for coal and gas outburst
With the increasing of destroyed-degree, the porosity increased, the anti-destruction capability reduced, the coal is more easily broken, then the risk of outburst is greater. 1 Introduction Coal is a solid material with abundant pores, and its pore structure characteristics are connected with occurrence state of the pore.
Journal of China Coal Society, 1987,12 (4) :51-57
Combustion Science and Technology, 2007, 13 (3):265-268

The stress of SEP exceeds the yield strength of material when the load reaches peak value.
Acknowledgements This work was financially supported by the Shanxi Natural Science Foundation (2011JQ7008), the Special Research Foundation (11JK0965) from the Education Institution of Shaanxi, and the Fundamental Research Foundation (JC1105) from Xi’an University of Architecture & Technology.
Journal of Nanjing university of technology (Natural Science edition). 2008, 30(1): 92-96.

The experiment material was eutectic Al-26%Si.
Acknowledgements This work was financially supported by the 12th Basic Research Project of Fok Ying Tung Education Foundation (121054), the Colleges and Universities Excellent Talents Project of Liaoning Province (LR2011003), the Application Basic Research Project of Shenyang (1081229-1-00), the Basic Research Project of Shenyang (F10-205-1-49), the Industrial Science and Technique Project of Shenyang (F11-033-2-00), the Industrial Science and Technique Project of Liaoning Province (2011221008).
Law, Journal of Electrostatics 51 (2001) 25-42

Numerical Calculation on Contact Stress Start Input force, structure parameters, material parameters and iterative precision Calculate coefficients C and D For i=0, e (0) =0, f (0) (e) =C/D, The search interval [0, 1.1] i=i+1, use the iterative method to calculate e (i+1) and f (i+1) (e) N Output e; call the program to calculate contact stress.
Cui: Design of Traction-planetary CVT (Henan science and Technology Press, Zhengzhou 1994).
Gao: Heavy Industry Science and Technology (1) (2006), p. 12.
Su: Journal of Shenyang Institule of Aeronautical Engineering (3) (2009), p. 5.