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Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes* Songlin Zhang1,a,Meilin Jin2,3,b, Huanchun Chen2,3,b 1Shandong Binzhou Animal Science ＆Veterinary Medicine Academy, Binzhou 256600, China 2Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China 3Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan430070, PR China ajlgzlzsl@qq.com,bjml8328@126.com Keywords: Deletion mutant; Homologous recombination; Pseudorabies virus (PRV); vaccine Abstract.
Materials and Methods A.Plasmid, cells and virus The parental virus, PRV Ea strain, a spreading strain, was originally isolated by our laboratory [8].
“Dissemination of wild-type and gC-, gE- and gI-deleted mutants of Aujeszky’s disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation,” Journal of General Virology, vol. 76, Aug. 1995, pp. 2063-2066, doi: 10.1099/0022-1317-76-8-2063

Because many for history carry records, material is unknown.
In 1989, Tongjing of Yubei Chongqing 5.4 on the Richter scale have detailed investigation material, more research in the earthquake.
Acknowledgements This work was financially supported by the Chongqing Municipal Science and Technology Project, Project No.
Earthquake journal, 2003, 25 (2): 199 ~ 204.

Sun, “Comparison of DC Pollution Flashover Performance of Various Types of Long String Insulators,” Journal of Chongqing University(Natural Science Edition), vol. 30, no. 7, pp. 42-45, 2007(in Chinese)
Conf. on Properties and Application of Dielectric Materials, pp. 260-263, Beijing, China,1988 [17] R.

As for energy price, it is difficult to the statistical data on energy price, we select raw material, fuel and power purchasing price index of Beijing to replace energy price.
Based on the previous research result, we use the efficiency measure from the stage 1 DEA estimations as the dependent variables, and determinants of energy efficiency as explanatory variables, we can estimate the following Tobit regression model: (7) Where, is the annual energy efficiency, is the ratio of industrial added value to GDP, is the proportion of coal consumption in total energy consumption, is the raw material, fuel and power purchasing price index of Beijing, and is Beijing’s R&D expenditure.
Acknowledgements This work was financially supported by the Beijing Social Science Foundation (11JGB071).
Zhou, B.W.Ang and K.L.Poh: European Journal of Operational Research Vol. 189 (2008), p.1-18

Thisconcept is named “self-matching fuzzy ball plugging”[12].Rigid material and flexible material use their own intensityto form a “wall” to resist the fluid into the formation, whilethe fuzzy balls take advantage of the pressure, temperatureand passage shape of the losing formation and combinetheir own properties of high viscosity and deformability toprevent the fluid losing.
Kunming University of Science and Technology, 2012
Application of Fuzzy-ball Drilling Fluids in Coalbed Methane Well Yan3U1-P[J].Journal of Chengde Petroleum College,2012,14(3)

Acknowledgments The work is performed within the state assignment of Russian Ministry of Education and Science (Assignment #2014/223, Project code #1347).
Lapin, Optimization of the process of nanoparticle fabrication by laser ablation of bulk targets in a liquid, Russian Physics Journal. 57, #3 (2015) In Print [12] R.
Lan, Nickel and cobalt nanoparticles produced by laser ablation of solids in organic solution, Materials Letters. 62 (2008) 1521-1524.

Experiment Materials.
Journal of Applied Polymer Science, Vol.102, 5848–5853, 2006 [7] Zhengsheng Wang.

In this paper, we take the 6408 series as an example, the material of inner race, outer race and balls is GCr15SiMn of which density is 7800kg/m3, Young's modulus is 2.08×105MPa and Poisson ratio is 0.29; the material of retainer is brass whose density is 8545kg/m3, Young's modulus is 1.06×10 5MPa and Poisson ratio is 0.324.
Journal of Automobile Engineering Science Vol. 216 (2002), p.455 [4] Di Sciuva M, Lomario D.A: Composite Structures 59 (2003), p. 155 [5] Manolis Papadrakakis, Nikos D Lagaros: Computer Methods in Applied Mechanics and Engineering 191(2002), p. 34 [6] Mainak Mazumdar, David W Coit, Kelvin MeBride: Quality Control and Applied Statistics 6 (2001), p.109

Experimental Materials Three kinds of lignite, coming from Ximeng of Inner Mongolia, Hami of Xinjiang and Indonesia, were employed in the experiment.
[2] Hongfeng Dong, Zengjie Yun, Yongfei Cao: Coal Technology, Vol.27(2008), p.122 (In Chinese) [3] Qinqin Guo, Zhen Yang, Jianwen Zhang: Boiler Technology, Vol.39(2008), p.45(In Chinese) [4] Yalin Qiu, Li Yang, BingfengYu: Energy Technology, Vol.27(2006), p.83(In Chinese) [5] Zhankuan Wang, Junzhong Li: North China Electric Power, Vol.3(2008), p.16 (In Chinese) [6] Qihong Nie, Shaozeng Sun, Zhengqi Li, et al: Journal of Combustion Science and Technology, Vol.7(2001), p.72 (in Chinese) [7] D.F.

Compared with angle steel towers, steel tube towers could bring the function of materials into full play and improve the stability of the structure.
Mechanical vibration.Beijing: Science Press. 341-382
Journal of Vibration and Shock 10, 60–64.