A Microfluidic Platform for Multi-Antigen Immunofluorescence Assays

Abstract:

Article Preview

Traditional cell-based assays such as cell immunoassay that utilizes plastic (chamber slides, dishes, microtiter plates), Magnetic bead, enzyme-linked immunsorbent assays (ELISA) [1], FACS cell sorting is labor intensive, time consuming, and requires a large numbers of cells or reagents. In this report, a microfluidic device integrated with cell culture, washing, fixation, and antigen-antibody reaction is presented for high-throughput immunoassay. Using this microfluidic device, each assay can be performed on a small number of cells and nanolitre or picolitre of reagents, this is particularly beneficial for rare or expensive cell types such as stem cells, or flow sorted cell populations. The capability of the microfluidic device was demonstrated for seeding human umbilical cord blood mesenchymal stem cells (UC-MSCs) in chambers and detecting the expression of surface markers (CD34, CD44, CD45, CD73, CD105, HLA-DR) by immunofluorescence assay.

Info:

Periodical:

Edited by:

Barry Tan

Pages:

200-205

DOI:

10.4028/www.scientific.net/AMM.108.200

Citation:

B. Y. Zhang et al., "A Microfluidic Platform for Multi-Antigen Immunofluorescence Assays", Applied Mechanics and Materials, Vol. 108, pp. 200-205, 2012

Online since:

October 2011

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.