Antibacterial Activities of Kappa-Carrageenan Oligosaccharides

Abstract:

Κ-carrageenan oligosaccharides were prepared by enzymatic degradation. The inhibiting activities of the prepared κ-carrageenan oligosaccharides against Escherichia coli (E.coli ), Staphylococcus aureus (S.aureus), Saccharomyces cerevisiae (S.cere), Penicillium citrinum (P.citr) and Mucor.sp were determined and compared by measuring the inhibitory zone diameter. The results indicate that all tested κ-carrageenan oligosaccharides have inhibiting activity against the five bacteria, but the inhibiting activities against Saccharomyces cerevisiae is higher than against any other four bacteria. The inhibiting concentration (MIC) of κ-carrageenan oligosaccharides are 1mg/ml, 5mg/ml, 20mg/ml, and antibacterial activity of κ-carrageenan oligosaccharides was inversely affected by concentration, with the highest activity at the concentration of 5mg/ml.

• Periodical: Applied Mechanics and Materials (Volume 108)
• Pages: 194-199
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.108.194
• Online since: October 2011
• Authors: Fei Fei Wang, Ziang Yao, Hai Ge Wu, Sheng Xia Zhang, Nan Nan Zhu, Xue Gai
• Keywords: Antibacterial, Saccharomyces cerevisiae, Κ-Carrageenan Oligosaccharides

References

[1] P. Nyvall, L. Maud, D. Richard, et al. Enzymatic Degradation of K-Carrageenan in Aqueous Solution, [J]. Biomacromolecules 2009, 10, 1757–1767.

[2] J. Yan, H.L. Zong, A.G. Shen, et al. Theβ-(1→6)branchedβ-(1→3)glucohexaose and its analogues containing anα-(1→3)-linked bond have similar stimulatory effects on the mouse spleen as Lentinan, [J]. Int J Immunop- harmacol, 2003, 3(13-14): 1861-1871.

DOI: 10.1016/j.intimp.2003.09.003

[3] J. Ning, W . Zhang, Y.T. Yi , et al. Synthesis ofβ-(1→6)-branchedβ-(1→3)glucohexaose and its analogues containing anα-(1→3)linked bond with antitumor activity, [J]. Bioorg Med Chem, 2003, 11(10): 2193-2203.

DOI: 10.1016/s0968-0896(03)00118-4

[4] H.M. Yuan, L.M. Song, X.G. Li, et al. Immunomodulation and antitumor activity of κ-carrageenan oligosaccharides, [J]. Cancer Letters, 2006, 243(2): 228-234.

DOI: 10.1016/j.canlet.2005.11.032

[5] H.M. Yuan, W.W. Zhang, X.G. Li, et al. Preparation and in vitro antioxidant activity of κ-carrageenan oligosaccharides and their over sulfated, acetylated and phosphorylated derivatives, [J]. Carbohydrate Research, 2005, 340 (4): 685-692.

DOI: 10.1016/j.carres.2004.12.026

[6] K. shida, M.K. Wierzba, T . Teruya, et al. Novel heparin sulfatemimetic compounds as antitumor agents, [J]. Chem Biol, 2004, 11(3): 367-377.

[7] X. Li, H.Q. Wang. Radio protective effect of carrageenan derived oligosaccharides on mice with radiation injury, [J]. Chinese Journal of Radiological Medicine and Protection, 2005, 25(2) : 116-117.

[8] Q.L. Cui, J.Y. Huang, L.J. Wang. Antibactrial test in viro and application of Shuang huanglian injection, [J]. Henan Journal of Husbandry and Veterinary Medicine, 2004, 35(1): 42-43.