Chiral Separation of Non-Natural Carboxylic Amino Acid by Capillary Electrophoresis with CD Derivations as Chiral Selective Material

Abstract:

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A capillary electrophoresis (CE) method for the separation of four kinds of enantiomers of non-natural carboxylic amino acid was built while hydroxypropyl-β-cyclodextrin (HP-β-CD) derivations as chiral selective material. Several different β-CD derivatives were tested for the chiral separation of non-natural carboxylic amino acid, and it was proved that HP-β-CD could show better chiral selectivity. The separation of enantiomers of amino acid was obtained by CE in a 50-μm i.d.×60 cm (effective length 45 cm) fused-silica capillary at 18 kV voltage, while 10 mM phosphate acted as running buffer and HP-β-CD served as selective material. The detective wavelength was set at 254 nm.

Info:

Periodical:

Edited by:

Han Zhao

Pages:

4126-4129

DOI:

10.4028/www.scientific.net/AMM.130-134.4126

Citation:

B. H. Li and B. J. Tian, "Chiral Separation of Non-Natural Carboxylic Amino Acid by Capillary Electrophoresis with CD Derivations as Chiral Selective Material", Applied Mechanics and Materials, Vols. 130-134, pp. 4126-4129, 2012

Online since:

October 2011

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$35.00

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