The design of multi-channel temperature acquisition system based on MSP430 and temperature sensor DS18B20 is presented in this paper. The simulation can be realized by using Proteus. The system has the following advantages: the structure of system is specific; the circuit is simple and it’s easy to control. The system can complete the acquisition and display of multi-channel temperature, it also has other functions such as transferring data to PC, etc. The simulation shows that the system is low cost, high reliability and high practical value.