In Silicon Cloning and Bioinformatics Analysis of an Eukaryotic Initiation Factor 4E Gene from Grimmia pilifera

Abstract:

GH425 gene comes from the Grimmia pilifera drought stress of cDNA library.In this experiment,we have got the full sequence of GH425NO.1 by E-cloning which using GH425 as gene probe,in Physcomitrella patens DNA Datebase.Through using ORFfinder to find out the longest ORF and design primer for it,then, validated the Physcomitrella patens by PT-PCR,and we have obtained corresponding band and proved that the result of silicon cloning is correct and the fragment is contained in Grimmia pilifera P.Beauv.Now,we know the sequence encodes Eukaryotic initiation factor 4E by Blastx,and analysis it with Bioinformatics.

• Periodical: Applied Mechanics and Materials (Volumes 138-139)
• Pages: 1132-1138
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.138-139.1132
• Online since: November 2011
• Authors: Wei Sha, Li Wu, Xiao Hong Song
• Keywords: Bioinformatic Analysis, Drought Stress, eIF4E, Grimmia pilifera, In Silicon Cloning

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