Production of a Fibrinolytic Enzyme from Coprinus comatus YY-20

Abstract:

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A strong fibrin-specific fibrinolytic enzyme was produced by liquid culture of Coprinus comatus YY-20. Soybean cake powder and sucrose as substrates resulted in the highest fibrinolytic activity. The optimal temperature for the production of the enzyme was found to be 21-25°C in the optimal medium (Soybean cake powder 50 g/L, sucrose 20 g/L). The optimal initial pH of the medium was found to be at the range of 5-8. The maximum fibrinolytic activity was observed at the stationary phase of cell growth. These results suggest that the culture liquid of Coprinus comatus YY-20 has potential as a source of physiologically active fibrinolytic enzymes.

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Periodical:

Edited by:

Honghua Tan

Pages:

1195-1201

DOI:

10.4028/www.scientific.net/AMM.138-139.1195

Citation:

X. L. Liu et al., "Production of a Fibrinolytic Enzyme from Coprinus comatus YY-20", Applied Mechanics and Materials, Vols. 138-139, pp. 1195-1201, 2012

Online since:

November 2011

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$35.00

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