Preparation and Characterization of Mn_o.5Zn_o.5Fe₂O₄ @Au Composite Nanoparticles and its Anti-Tumor Effect on Glioma Cells

Abstract:

To explore the preparation method and characters of a new gold nanoshells on maganese-zinc ferrite (Mn_o.5Zn_o.5Fe₂O₄@Au) composite nanoparticles. Mn_o.5Zn_o.5Fe₂O₄@Au nanoparticles with core/shell structure were synthesized by reduction of Au³⁺ with trisodium citrate in the presence of Mn_o.5Zn_o.5Fe₂O₄ magnetic nanoparticles (MZF-NPs) prepared by improved co-preciption with the character of superparamagnetism and detected by transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD), energy dispersive spectrometry (EDS) and Marven laser particle size analyzer.Thermodynamic test was used to observe temperature change of various doses of Mn_o.5Zn_o.5Fe₂O₄@Au nanoparticles. The cytotoxicity of the Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles in vitro was tested by the MTT assay. The therapeutic effect of Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles combined with magnetic fluid hyperthermia (MFH) on human glioma cells were evaluated in vitro by an MTT assay.The results indicated that the Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles were prepared successfully. The core/shell particles were spherical with exact average diameter of them was 66.9nm.EDS showed each Mn_o.5Zn_o.5Fe₂O₄@Au nanoparticle contained Mn, Zn, Fe, O and Au elements, and this proved Au had successfully attached to Mn_0.5Zn_0.5Fe₂O₄.The result of thermodynamic test showed that Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles could serve as a heating source under alternating magnetic field (AMF) exposure leading to reach their steady temperature (40-45°C). Moreover, Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles didn’t show cytotoxicity in vitro. The therapeutic result reveals that Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles can significantly inhibit the growth of glioma cells.The conclusion was that the self-prepared Mn_o.5Zn_o.5Fe₂O₄@Au composite nanoparticles had strong magnetic responsiveness and good power absorption capabilities in the high frequency AMF,then they could suggested to be useful for glioma hyperthemia. Mn_o.5Zn_o.5Fe₂O₄ @Au composite nanoparticles can not only be directed to tumor region in a given magnetic field more exactly but also produce marked thermotherapy.

• Periodical: Applied Mechanics and Materials (Volumes 138-139)
• Pages: 907-913
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.138-139.907
• Online since: November 2011
• Authors: Yun Tao Li, Jing Liu, Li Wang, Jia Zhang, Zi Yu Wang, Yue Jiao Zhong, Dong Sheng Zhang, Zhi Qiang Gao
• Keywords: Characterization, Glioma Cells, Mn_o.5Zn_o.5Fe₂O₄@Au Hyperthermia, Preparation

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