The Effect of Thermal Contact Resistance on Heat Management in a Shuttling PCR System

Jyh Jian Chen; Wei Hua Chen; Yi Shiang Shie

doi:10.4028/www.scientific.net/AMM.284-287.1941

Paper Titles

Robust Adaptive Trajectory Control for an Omnidirectional Vehicle
p.1919

Studies on the Exchangeability of Different APT Interpreters for 5-Axis Machine Tool Applications
p.1924

Synthesis of Two-Degree-of-Freedom Rotational Decoupled Manipulators of a 2R-Type Branch
p.1929

System Identification and Robust Control of a Pneumatic Muscle Actuator System
p.1936

The Effect of Thermal Contact Resistance on Heat Management in a Shuttling PCR System
p.1941

The Fault Tolerant Output Selector Based on Fault-Detection Considering Realistic Fault Modes for Pedal Simulator of Brake-by-Wire System
p.1946

The Nonexistence of Three Degrees of Freedom Rotational Fully Decoupled Parallel Mechanism
p.1951

The Performance Analysis of an AKF Based Tightly-Coupled INS/GNSS Sensor Fusion Scheme with Non-Holonomic Constraints for Land Vehicular Applications
p.1956

The Squeeze Film Damping Effect on Dynamic Responses of Micro-Electromechanical Resonators
p.1961

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 284-287The Effect of Thermal Contact Resistance on Heat...

The Effect of Thermal Contact Resistance on Heat Management in a Shuttling PCR System

Abstract:

A novel shuttling polymerase chain reaction (PCR) system is assembled to make temperature uniform in the reaction chamber. The chamber is oscillated by a servo motor and contacted with three different isothermal zones to complete several thermal cycles. The home-made computer code is utilized to investigate the influences of operational parameters on the temperature inside the chamber. Numerical results show that the contact resistances between the heating blocks and the reaction chamber dominate the temperatures inside the PCR chamber. In this work a PCR system that is composed of the PID controller, the moving stage, three aluminum blocks for three different isothermal zones and a reaction chamber is also developed. Experimental results demonstrated that the stability of this shuttling PCR system is confirmed. And results show that DNA templates provided with the yT&A® cloning vector are amplified successfully in this PCR system.

You might also be interested in these eBooks

Innovation for Applied Science and Technology

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 284-287)

Pages:

1941-1945

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.284-287.1941

Citation:

Cite this paper

Online since:

January 2013

Authors:

Jyh Jian Chen, Wei Hua Chen, Yi Shiang Shie

Keywords:

Contact Resistance, PCR, PID Controller, Shuttling

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] M. Northrup, M.T. Ching, R.M. White and R.T. Wltson: Proceedings of the 7th International Conference of Solid-State Sensors and Actuators, Transducers '93 (1993), p.924.

Google Scholar

[2] M.U. Kopp, A.J.D. Mello and A. Manz: Science Vol. 280 (1998), p.1046.

Google Scholar

[3] Y. Li, D. Xing and C. Zhang: Anal. Biochem. Vol. 385 (2009), p.42.

Google Scholar

[4] Z. Chen, S. Qian, W.R. Abrams, D. Malamud and H.H. Bau: Anal. Chem. Vol. 76 (2004), p.3707.

Google Scholar

[5] J.J. Chen and Y.T. Yang: Appl. Therm. Eng. Vol. 31 (2011), p.2797.

Google Scholar

[6] J.P. Holman: Heat Transfer, 10th ed. (McGraw-Hill, New York 2009).

Google Scholar

[7] Y.A. Cengel: Heat Transfer: A Practical Approach, 2nd ed. (McGraw-Hill, New York 2002).

Google Scholar