In order to conduct the cleaning of spilled oil, to protect the marine ecological balance and to prevent the damage to marine environment by oil spill, the simulation of oil spill in deep water with the wave motion, wind and current was built by the user defined function and the volume of fluid in FLUENT. The dispersion and diffusion of oil spill in deep water was simulated dynamically. Also the oil spilling trajectories under the conditions of different current velocities, wavelengths and wind velocities were compared and analyzed, respectively. The results showed that the current, wind and wave decided the location and areas of oil films on the sea surface. The water depth influenced by the wave which increased with the increasing wavelength. Meantime, the areas of oil films on sea surface increased with current velocity decreasing, while the distance between the oil film and the source of oil increased. With the wind velocity increasing, the extended length of oil dispersion underwater and the oil film area on surface increased.