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Response of Alfalfa (Medicago sativa L.) Bud Seedling to Saline and Alkaline Stress under Water Culture Conditions

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Abstract:

Bud seedlings were used to study short-term effect of different saline and alkaline concentrations on alfalfa root and stem growth. Two neutral salts (NaCl and Na2 SO4) and 2 alkaline salts (NaHCO3 and Na2 CO3) were mixted at 9:1 mole ratio, respectively, to imitate typical saline and alkaline environments. 8 saline concentrations: 0, 10, 20, 40, 60, 80, 100, 120 mmol/L, and 7 alkaline concentrations: 0, 2, 5, 10, 20, 40, 50 mmol/L were selected to determine the stress effects on 5-day, 2-cm bud seedlings. The results indicated that bud seedlings’ root length increased firstly and then decreased as the increase of stress concentrations. On condition of neutral salt stress, 40 mmol/L treatment showed optimum root length, which was 102% times higher than CK, with significant difference (P<0.05). Under alkaline salt stress, 5 mmol/L treatment showed optimum root length, which was 156% times higher than the CK, with significant difference (P<0.05). The effects of the stresses on stem length surpassed root length. And the inhibitory effect of alkaline salt stress on seedlings’ growth in early stage surpassed neutral salt stress.

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Periodical:

Applied Mechanics and Materials (Volume 310)

Pages:

33-37

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.310.33

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Online since:

February 2013

Authors:

Jian Feng Li, Shu Qing Zhang, Shang Li Shi, Ping Hui Huo

Keywords:

Alkaline Stresses, Bud Seedling, Saline Stresses, Seedling Growth, Water Culture

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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