Bio-manufacture is an interdisciplinary research area, which is based on the development of rapid prototyping technology, biomaterial and biomedicine technologies. It brings a new revolution in manufacturing science. Producing scaffolds of high performance is the purpose of bio-manufacture. For many rapid prototyping, which is suitable for bio-manufacture, the core difference lies in their enable technology. Extrusion-based rapid prototyping technology is an important branch of rapid prototyping, and each system has different nozzle device. In this paper, through in-depth research and analysis of structure, performance and materials used of spray head in the market for bio-manufacture, it can provide scientific basis for selecting more suitable spray head of rapid prototyping for bio-manufacture.