Application of Porous Chitosan/Gelatin Bone Scaffolds Used in Bone Tissue Engineering

Abstract:

Chitosan (CS) and gelatin (G) both have good biocompatibility and biodegradation, qualifying them for use in tissue engineering. In this study, CS and G are blended with different ratios to make the mixture solution, and then freeze-dried to form three-dimensional porous CS/G bone scaffolds. The surface, cross-section, porosity, and pore size of the resulting bone scaffolds are observed and analyzed. According to the experimental results, the addition of gelatin gives the CS/G bone scaffolds morphology with few pores. As can be seen from SEM observation, there are linear pores in the cross-section. In addition, with a larger quantity of gelatin, the CS/G bone scaffolds have a lower porosity.

• Periodical: Applied Mechanics and Materials (Volumes 365-366)
• Pages: 1050-1053
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.365-366.1050
• Online since: August 2013
• Authors: Ching Wen Lou, Shih Peng Wen, Hsiu Ying Chung, Chao Tsang Lu, Jia Horng Lin
• Keywords: Bone Scaffolds, Chitosan, Gelatin, Porous

References

[1] I.V. Yannas, Tissue regeneration by use of collagen-glycosaminoglycan copolymers, Clin Mater. 9 (1992) 179-187.

DOI: 10.1016/0267-6605(92)90098-e

[2] S.F. Hulbert, F.A. Young, R.S. Mathews, J.J. Klawitter, C.D. Talbert, F.H. Stelling, Potential of ceramic materials as permanently implantable skeletal prostheses, J Biomed Mater Res . 4 (1970) 433-456.

DOI: 10.1002/jbm.820040309

[3] J.J. Klawitter, J.G. Bagwell, A.M. Weinstein, B.W. Sauer, An evaluation of bone growth into porous high density polyethylene, J Biomed Mater Res. 10 (1976) 311-323.

DOI: 10.1002/jbm.820100212

[4] N.Y. Yuan, Y.A. Lin, M.H. Ho, D.M. Wang, J.Y. Lai, H.J. Hsieh, Effects of the cooling mode on the structure and strength of porous scaffolds made of chitosan, alginate, and carboxymethyl cellulose by the freeze-gelation method, Carbohydrate Polymers. 78 (2009).

DOI: 10.1016/j.carbpol.2009.04.021

[5] K. Rezwan, Q.Z. Chen, J.J. Blaker, A.R. Boccaccini, Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering, Biomaterials. 27 (2006) 3413-3431.

DOI: 10.1016/j.biomaterials.2006.01.039

[6] J.S. Mao, L.G. Zhao, Y.J. Yin, K.D. Yao, Structure and properties of bilayer chitosan-gelatin scaffolds, Biomaterials. 24 (2003) 1067-1074.

DOI: 10.1016/s0142-9612(02)00442-8

[7] W.M. Parks, Y.B. Guo, A casting based process to fabricate 3D alginate scaffolds and to investigate the influence of heat transfer on pore architecture during fabrication, Mater. Sci. Eng., C. 28 (2008) 1435-1440.

DOI: 10.1016/j.msec.2008.03.013