Selenylation, Characterization and Cytotoxic Activity of Seleno-Astragalus Polysaccharide

Abstract:

This paper investigated the selenylation, characterization and cytotoxic activity of seleno-Astragalus polysaccharide. Firstly, Se-APS was synthesized under reaction time at 8 h, reaction temperature at 80 °C, ratio of Na₂SeO₃ to APS at 1.0 g/g and water bath shaking rate at 40 r/min. Then, a series of experiments were designed to investigate the characterization and cytotoxicity of Se-APS. The result indicated that the characterization of Se-APS was significantly different from APS, except for X-ray diffraction. Additionally, MTT assay conformed that Se-APS could significantly inhibit the proliferation of human breast cancer MCF-7 cells in dose-dependent and time-dependent manners.

• Periodical: Applied Mechanics and Materials (Volumes 411-414)
• Pages: 3146-3149
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.411-414.3146
• Online since: September 2013
• Authors: Yu Bin Ji*, Fang Dong, Miao Yu
• Keywords: Characterization, Cytotoxicity, Seleno-Astragalus Polysaccharide(Se-APS)

References

[1] State Pharmacopoeia Commission: Pharmacopoeia of the People's Republic of China (a), (Chemical Industry Press, Beijing, China 2005).

[2] F. Wu, X Chen: Zhong Yao Cai Vol. 27(2004), pp.232-234.

[3] Q.J. Yan, L.J. Hang, Z.Q. Jiang, et al: Food Science Vol. 25(2004), pp.27-30.

[4] D.J. Xu, Q. Xia, J.J. Wang, et al: Molecules Vol. 13(2008), pp.2408-2415.

[5] P.A. Tsuji, S. Naranjo-Suarez, B.A. Carlson, et al: Nutrients Vol. 3(2011), pp.805-817.

[6] D. Shang, Y. Li, and C. Wang: Oncology Reports Vol. 25(2011), p.267–272.

[7] J. Zhang, F.X. Wang, Z.W. Liu, et al: Nat. Prod. Res. Vol. 23(2009), p.1641–1651.

[8] Y.J. Huang, B.A. Song, L.H. Jin, et al: Journal of Biology, Vol. 26(2009), pp.13-16.