Construction of Transgenic Drosophila Lines as a Cyclind Expression Reporter System

Heng Jiang Li

doi:10.4028/www.scientific.net/AMM.411-414.3210

Paper Titles

Research Progress on the Anti-Leukemia Effect of Lycorine
p.3193

Study on Modified Atmosphere of Newhall Navel Orange by Different Packing Films
p.3197

Study on Production of Polypeptide Milk by Enzymolysis Technology
p.3201

Research on Enzymatic Hydrolysis of Whey Protein
p.3205

Construction of Transgenic Drosophila Lines as a Cyclind Expression Reporter System
p.3210

Research on the Extraction Technology of Alkaloids in Natural Medicine
p.3218

The Research on the Medicinal Value of Amaryllidaceae Plants
p.3223

Optimizing Discriminant Model for Improved Classification of Protein
p.3227

Research on Structural Modification and Structure-Activity Relationship about Anti-Tumor of Polysaccharides from Plants
p.3232

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 411-414Construction of Transgenic Drosophila Lines as a...

Construction of Transgenic Drosophila Lines as a Cyclind Expression Reporter System

Abstract:

A 1188bp regulatory sequence upstream of Cyclin D promoter is amplified by PCR, using Drosophila genome as a template, and then cloned into PcaSpeR-AUG-βgal plasmid for injecting into the W¹¹¹⁸ line. We obtain several independent transgenic lines with mini-white as a selection marker. After mapping and balancing, immunostaining, the transgenic embryos perform a pattern which is similar to the 11407 line. The results show that these transgenic lines can mimic the endogeneous Cyclin D expression pattern in Drosophila. Thus a lacZ reporter system is constructed for analyzing the Cyclin D function.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 411-414)

Pages:

3210-3217

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.411-414.3210

Citation:

Cite this paper

Online since:

September 2013

Authors:

Heng Jiang Li*

Keywords:

Cyclin D, lacZ Reporter System, Microinjection, Transgenic Drosophila

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] Baldin,V., Lukas, j., Marcote M.J., Pagano,M. and Draetta,G. (1993) Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev., 7, 812-821.

DOI: 10.1101/gad.7.5.812

Google Scholar

[2] Frei C. (2004) Cyclin D/Cdk4: New insights from Drosophila. Cell Cycle 3, 558-60.

DOI: 10.4161/cc.3.5.867

Google Scholar

[3] Frei C, Edgar BA. (2004) Drosophila cyclin d/Cdk4 requires hif-1 prolyl hydroxylase to drive cell growth. Dev Cell 6, 241-51.

DOI: 10.1016/s1534-5807(03)00409-x

Google Scholar

[4] Martin P Zeidler1, Erika A Bach1 and Norbert Perrimon (2000) The roles of the Drosophila JAK/STAT pathway. Oncogene 19, 2598 ± 2606.

DOI: 10.1038/sj.onc.1203482

Google Scholar

[5] Claas A. Meyer, Henning W. Jacobs, and Christian F. Lehner1(2002). Cyclin D-Cdk4 is not a master regulatorof cell multiplication in Drosophila embryos. Current Biology. 12, 661–666.

DOI: 10.1016/s0960-9822(02)00770-4

Google Scholar

[6] Meyer, C. A., H. W. Jacobs, S. A. Datar, W. Du, B. A. Edgar et al., Drosophila Cdk4 is required for normal growth and is dispensable for cell cycle progression. EMBO J. 19: 4533–4542.

DOI: 10.1093/emboj/19.17.4533

Google Scholar

[7] Ganter, B., S. Fu and J. S. Lipsick, 1998 D-type cyclins repress transcriptional activation by the v-Myb but not the c-Myb DNA binding domain. EMBO J. 17: 255–268.

DOI: 10.1093/emboj/17.1.255

Google Scholar

[8] Horstmann, S., S. Ferrari and K. H. Klempnauer, 2000 Regulation of B-Myb activity by cyclin D1. Oncogene 19: 298–306.

DOI: 10.1038/sj.onc.1203302

Google Scholar

[9] Bienvenu, F., H. Gascan and O. Coqueret, 2001 Cyclin D1 represses STAT3 activation through a Cdk4-independent mechanism. J. Biol. Chem. 276: 16840–16847.

DOI: 10.1074/jbc.m100795200

Google Scholar

[10] Reutens, A. T., M. Fu, C. Wang, C. Albanese, M. J. McPhaul etal., 2001 Cyclin D1 binds the androgen receptor and regulates hormone-dependent signaling in a p.300/CBP-associated factor (P/CAF)-dependent manner. Mol. Endocrinol. 15: 797–811.

DOI: 10.1210/mend.15.5.0641

Google Scholar

[11] Jan Emmerich, Claas A. Meyer, Aida Flor A. de la Cruz, Bruce A. Edgar and Christian F. Lehner（2004）Cyclin D Does Not Provide Essential Cdk4-Independent Functions in Drosophila. Genetics 168, 867-875.

DOI: 10.1534/genetics.104.027417

Google Scholar