Molecular Differentiation of Nontuberculous Mycobacterium Isolated from Different Animals

Abstract:

To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of 16SrDNA and the 65-kDa heat-shock protein gene (hsp65 gene). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals are an important environmental source of mycobacteria and the combined application of 16SrDNA and hsp65 sequencing was more reliable to accurately identify mycobacteria present in animals.

• Periodical: Applied Mechanics and Materials (Volume 421)
• Pages: 300-303
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.421.300
• Online since: September 2013
• Authors: Chun Fang Wang, Hua Rui Qi, Xiu Yun Jiang, Fei Fei Chen, Hong Xia Ma, Chun Feng Wang, Ai Dong Qian
• Keywords: 16SrDNA, hsp65 Gene, Molecular Differentiation, Nontuberculous Mycobacterium

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