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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 421Construction of Eukaryotic Expression Vector and...

Construction of Eukaryotic Expression Vector and Expression on Mycobacterium bovis ag85a and mpb70 Genes

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Abstract:

Based on the polymerase chain reaction (PCR), ag85a and mpb70 Fusion gene of Mycobacterium bovis were ligated and cloned into pMD18-T, then recombinant plasmid pMD-85a-70 was constructed. pMD-85a-70 and pVAX1-BMS were digested by double enzymes HindIII and EcoRI, the purified ag85a-mpb70 was subcloned into pVAX1-BMS, then recombinant plasmid pVAX1-BMS-85a-70 was constructed and transient expressed in Marc145 cell. These results laid solid foundations for further studies on ag85a-mpb70.

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Information Technology for Manufacturing Systems IV

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Periodical:

Applied Mechanics and Materials (Volume 421)

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308-312

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.421.308

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Online since:

September 2013

Authors:

Shuang Hou, Chun Fang Wang, Yan Ru Zheng, Jia Ning Guan, Jia Ming Lin, Da Ming Gao, Yun Hang Gao, Hong Xia Ma, Xiu Yun Jiang

Keywords:

ag85a Gene, Eukaryotic Expression, Fusion Gene, mpb70 Gene, Mycobacterium bovis

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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