Digital Image Restoration in Microscopic Measurement

Abstract:

The requirement of the accuracy of microscopic measurements becomes more and more high, and traditional methods have been unable to meet the demands. Digital image deconvolution techniques are applied in microscopic measurement. By fabricating a pointolite, the point spread function of the optical system is measured, and then the geometric blurring in traditional microscopy is removed by using the maximum likelihood estimation algorithms and iteration threshold segmentation algorithms. The technique is applied to measure the total content of perlite and spheroidal graphite in spheroidal graphite iron accurately and easily, and then to measure the area of a scratch scaled in 10 microns on a medical department of orthopedics plates. The technique makes great sense in the development of corresponding measurement.

• Periodical: Applied Mechanics and Materials (Volume 421)
• Pages: 421-426
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.421.421
• Online since: September 2013
• Authors: Hua Yue Du, Yong Qian, Cheng Guo Wu, Yan Hua Huang, Xia Li
• Keywords: Mage Deconvolution, Microscopic Measurement, Point Spread Function

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