Applied Mechanics and Materials Vols. 423-426

Abstract: To study the injury mechanism of stress wave from temporary cavity in gelatin, a stress wave propagation model is established. Based on experimental phenomena, gelatin around the trajectory is divided into fail region and elastic region. In the fail region, gelatin is considered to be incompressible and loop stress is zero. Gelatin in the elastic region is assumed to be plane-strain. Moving equations in the two regions are built. A 4.8 mm steel sphere is fired into gelatin at 720 m/s. Displacements of the temporary cavity are obtained from the experiment. By solving the moving equations with boundary conditions, amplitude of cavity pressure, radial stress distribution in gelatin are obtained. The theoretical results can be used to explain stress waves produced by temporary cavity.

Abstract: The isolate MP-63280 was isolated from imported soybean in Shanghai port by traditional PDA method. The isolate grew fast, produced typical black microsclerotia. Universal primers and special primers were used to amplify and sequence from MP-63280. Compared with the identity of rDNA sequence of Macrophomina phaseolina from NCBI GenBank databases by blast method, the result showed that its homology was up to 99%. Pathogenicity tests showed that soybean, cotton and sweet potato were infected by MP-63280. Based on morphological characteristics, molecular and pathogenicity test results, the isolate was identified as Macrophomina phaseolina (Tassi) goid. The effective methods to prevent the fungi from spread and transimission are to strengthen inspection and quarantine.

Abstract: Objective: To investigate the expression of p53 gene in gastric cancer tissue and its correlation with bcl-2 and bax protein expression in relationship and discuss the significance of their correlation in clinic. Methods: Pathological specimens from 100 gastric cancer patients with complete medical data and 24 normal lung tissue specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of bcl-2, bax and p53 protein. Results: p53 expression was positively correlated with bcl-2 expression (Pearson's R =0.491, P <0.05). The positive expression of p53 was no significantly correlated with bax expression (P> 0.05). The positive rate of p53 expression in the well-differentiated gastric cancer tissues was 17.6%, the positive rate in the differentiated tissues was 90.9%, the positive rate in the poorly differentiated tissues was 72.7%, and There was a significant difference in the expression of p53 in the three tissues (P <0.05). The expression of p53 in the TNM staging showed that the positive rate in stage Iand IIwas 27.3%, the positive rate in stage III and stage IVwas 89.3%, the difference between them was significant (P <0.05). Conclusions: The expression of p53 gene has an obvious clinical significance for the assessment of degree of malignancy and the prognosis of gastric cancer. Bcl-2 and p53 gene expression could jointly promote the development of gastric cancer in a synergistic way by acting on different stages of apoptosis, or bcl-2s participating in p53-induced mitochondria-mediated apoptosis signaling pathways.

Abstract: Objective: In this study, immunohistochemical streptavidinbiotin-peroxidase (S-P method) was used to detect the correlation of gene proteins related to apoptosis, such as Bcl-2 and Survivin in colorectal carcinoma. Methods: Pathological specimens from 46 colorectal cancer patients and 8 normal tissues around the tumor specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of Bcl-2 and Surviving protein. Results: In 25 cases of Bcl-2 positive expression, there were 24 cases of Survivin positive expression, accounting for 96%; while there were 8 cases of Survivin positive expression in 21 cases of Bcl-2 negative expression, accounting for 38.1%, showing the expression of Bcl-2 and the expression of Survivin was positively correlated (Pearsons R= 0.627, P<0.01). In 15 cases of well-differentiated colorectal cancer tissues, there were 6 Cases of Survivin positive expression, and the positive rate was 40%; in 17 cases of moderately differentiated colorectal cancer tissues, there were 17 cases, and the positive rate was 100%; in 14 cases of poorly differentiated colorectal cancer tissues, there were 9 cases, and the positive rate was 64.29%. The statistical analysis showed that the Survivin positive expression in the three colorectal cancer tissues presented a significant difference (P <0.01). Results: The positive expression of Survivin protein was directly correlated to that of Bcl-2, which suggested that both of them acted on different stages of apoptosis to promote jointly the development of colorectal cancer in a synergistic way.

Abstract: The aim of this work to evaluate the influence of 1-MCP, O₃ and 1-MCP+O₃ to Dendrobium officinale. 1-MCP could inhibit plants respiration rate, and it was 14.57 mg CO₂·kg·FW·h-for 7 days which lower than CK and O₃ had similar inhibitory effect to respiration.1-MCP, O₃ could inhibit weight loss and O₃ was more significant than 1-MCP, but 1-MCP+O₃ treatments weight loss was similar to CK for 60 days, however 1-MCP+O₃ had significant effect on color change, it could inhibit color change during storage for plants.

Abstract: Regenerated bamboo fiber was prepared with green solvent. The rheological behavior of the spinning solution was investigated and a dry-jet wet spinning process was applied for making bamboo fiber. The results showed that spinning solution belonged to the typical shear thinning fluid, and it is found that the content had influence on the apparent viscosity of solution. In addition, the regenerated bamboo fiber had cellulose II crystal structure. and the orientation and crystallinity of fiber increased with the increasing of draw ratio, thereby the mechanical properties of fiber improved.

Abstract: Objective: To investigate anti-aging effects of deoxyschizandrin in rats and its mechanisms. Methods: D-galactose (120mg • kg-1 • d-1) was subcutaneously injected daily for 6 weeks to build a rat aging model, and deoxyschizandrin (50,100 and 200 mg • kg-1 • d-1) was consecutively administered daily for six weeks from the second day. Morris water maze was used for the observation of learning and memorizing abilities of the rats; spectrophotometry was applied to detect malondialdehyde (MDA) contents, superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px) activities, Na+-K+-ATPase and Ca2+-ATPase activities in the rats’ brain tissues. Results: The results showed that moderate-dose and high-dose deoxyschizandrin could improve learning and memorizing abilities of D-galactose-induced aging rats, enhance SOD, GSH-Px, Na+-K+- and Ca2+-ATPase activities, and reduce MDA levels in the rats’ brain tissues significantly (P <0.05 or P <0.01). Conclusion: deoxyschizandrin can improve rats’ learning and memorizing abilities, and its mechanisms may be associated with the increase in antioxidant enzymes such as SOD and GSH-Px in the body, the decrease in the production of MDA, the enhancement of ability to scavenge free radicals and inhibit lipid peroxidation to protect the brain cells from the damage by free radicals.

Abstract: Oil-in-water (O/W) nanoemulsion of Macadamia integrifoblia oil was prepared. The nanoemulsion with the minimum diameter (22.13 nm) would be gained at the ratio of surfactant (Tween-80): cosurfactant (olive oil): oil phase (ethanol)=9:2:1, 86.4% distributing between 10~30 nm, spherical. The Macadamia integrifoblia oil nanoemulsion was still clarity, homogeneous and stable after the stability tests.

Abstract: This study was aimed to investigate the protective effect of lignanoid against doxorubicin-induced cardiotoxicity in rats. Doxorubicin were administered at the dosage of 5 mg/kg once a week, ip for a period of 5 consecutive weeks. Lignanoid were administered at the dosages of 25, 50 and 100mg/kg, po by gavage for 7 consecutive days in a week for 5 weeks. Lignanoid at the doses of 50 and 100 mg/kg significantly reduced CK-MB, NO and LDH and increased GST levels in the doxorubicin-treated group. Thus, lignanoid ameliorated doxorubicin-induced cardiotoxicity by reducing oxidative stress, abnormal cellular morphology in rat. This study indicates the protective effect of lignanoid against doxorubicin-induced cardiotoxicity.

Abstract: The eri-silk was degummed by Alkaline protease,the chemical composition of the eri-silk was analyzed and the internal structure of the retting eri-silk was analyzed by infrared spectral, also the vertical form of the was tested by electron microscopy and using thermal analysis to study the thermal properties of the eri-silk. The results showed that the highest content in the eri-silk was alanine, followed by acetic acid; The heat resistance of the eri-silk was worse than the soybean protein fiber and wool, the the surface of the degumming eri-silk was smooth, there was obvious dents between two silk fibroin; the structure of the eri-silk fibroin contain α-helix and β-folding structure which is the most main structure, there is distinguishing peak characteristics of AmideI at wave 1661.