Oxidative Stress Effect on Testicular Germ Cells and Liver Cells in Mice Induced by Dibutyl Phthalate

Abstract:

Dibutyl phthalate (DBP) as a commonly used plasticizer can be detected in many environmental medium. In this study oxidative damage effect on testicular germ cells and liver cells in mice induced by DBP were investigated via vitro test. Testicular germ cells and liver cells of mice were incubated with medium containing DBP in different concentrations (0μmol/L,10μmol/ L,50μmol/L,100μmol/L), after one hour thiobarbituric acid assay and xanthine oxidase assay were used to measure the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) respectively.The results showed that the levels of MDA in testicular germ cells and liver cells increased gradually with the increase of DBP concentrations, while compared with the control group, the activities of SOD declined significantly at dosage of 50μmol/L and 100μmol/L. These results suggested that DBP could cause lipid peroxidation in testicular germ cells and liver cells of mice in vitro. Oxidative damage may be one of its toxic mechanisms. It would provide an experimental foundation for security application of DBP.

• Periodical: Applied Mechanics and Materials (Volumes 448-453)
• Pages: 241-244
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.448-453.241
• Online since: October 2013
• Authors: Jing Zhang, Huan Li, Jing Hong Yu*
• Keywords: DBP, Liver Cells, Malondialdehyde, Oxidative Stress, Testicular Germ Cell

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DOI: 10.1038/sj.onc.1208207