Development of a Temperature Control System Based on DSP for a Real-Time PCR Instrument

Hao He; Ji Hong Feng; Kai Xiang Li

doi:10.4028/www.scientific.net/AMM.462-463.549

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 462-463Development of a Temperature Control System Based...

Development of a Temperature Control System Based on DSP for a Real-Time PCR Instrument

Abstract:

In this paper, we develop a temperature control system based on DSP chip TMS320F28335 for a small real-time PCR instrument. In the system, PWM waves generated by the DSP passes through power amplifier circuit to drive the peltier, and a pt100 is used as a temperature sensor to build a Wheatstone bridge sensing circuit. The temperature signal from the pt100 is converted into voltage signal. Then the voltage signal goes through the A/D converted module and the Position PID algorithm to adjust the duty cycle of the PWM waves. Experimental results show that the system's rising and cooling rate can reach 4°C/s with an accuracy of 0.2°C.

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Periodical:

Applied Mechanics and Materials (Volumes 462-463)

Pages:

549-552

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.462-463.549

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Online since:

November 2013

Authors:

Hao He, Ji Hong Feng*, Kai Xiang Li

Keywords:

DSP, Real-Time PCR, Temperature Control

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* - Corresponding Author

References

[1] R.K. Saiki, S. Scharf, F. Faloona, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 1985, 230(4732): 1350-1354.

DOI: 10.1126/science.2999980

Google Scholar

[2] M. Schutten, B. Hoogen, M.E. Ende, et al. Development of a real-time quantitative RT-PCR for the detection of HIV-2 RNA in plasma. J Virol, 2000, 88: 81-87.

DOI: 10.1016/s0166-0934(00)00177-4

Google Scholar

[3] M.F. Willard, J.W. Stephen, E.V. Kent. Quantitative RT-PCR: pitfall and potential. Bio Techniques, 1999, 26(1): 112-125.

Google Scholar

[4] K.J. Liva, S.J.A. Flood, J. Marmaro, et al. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic-acid hybridization. PCR Methods and Applications, 1995, 4(6): 357-362.

DOI: 10.1101/gr.4.6.357

Google Scholar

[5] X. M. Li, Y. L. Zhang. A Temperature Control System for the Array of Micro-reactor PCR Chips Based on the Chips of Measurement and Control Model [J]. Mechatronics, 2006, 7: 19-23.

Google Scholar

[6] Texas Instruments Incorporate. TMS320X281x, 280x Peripherals Reference Guide (Rev. B) [R]. (2004).

Google Scholar

[7] Texas Instruments Incorporate. TMS320F2833X Analog to Digital Converter (ADC) Module Reference Guide [R]. (2007).

Google Scholar