Determination of Antioxidant Activity In Vitro from Hyptis rhomboidea Mart. et Gal. Ethanol Extracts

Abstract:

Antioxidants are the chemical substances that reduce or prevent oxidation. The present study aimed to assess in vitro antioxidant activities of ethanol extracts Hyptis rhomboidea. DPPH, ABTS and FRAP assays were used for characterization of antioxidant activities of several extracts. Eventually, the promising extract was the ethyl acetate (EAc) extract, The analyzed extracts exhibited that antioxidant activities varied considerably as function of extracts. The highest DPPH• scavenging ability was found in Y_6-8，which was divided from EAc by silica column, the lowest IC₅₀ value (6.9μg/ml), followed by Y_6-9 (11.7μg/ml) and the TEAC of Y_6-8 and Y_6-9were greater than positive control trolox. The same tendency was observed using ABTS and FRAP assays. These results suggest that the H. rhomboidea shows a better antioxidant activity, especially in the EAc extract and these results would be a guide in the selection of potential cultivars which are used in food and chemical industry and fresh market.

• Periodical: Applied Mechanics and Materials (Volume 472)
• Pages: 824-828
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.472.824
• Online since: January 2014
• Authors: Xi Feng Li, Lu Tang, Nan Nan Zheng, Shuo An, You Xu Wang, Wei Xiao Zhang, Ya Nan Hu
• Keywords: ABTS, Antioxidant Activity, DPPH, Ethanol Extracts, FRAP, Hyptis rhomboidea

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