Design and Performance of a High-Efficiency Cell-Electrofusion Device

Abstract:

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A microfluidic cell-electrofusion device was developed for high fusion efficiency and automatic manipulation. It included an experimental platform, an electric signal generator and a microchip. Microstructure and controlling signal were elaborately chosen in order to realize precise cell manipulation, alignment and electrofusion. Compared with the traditional cell-electrofusion device, this device has some outstanding advantages. For example, short distance between two counter-microelectrodes will reduce the required voltage. A large number of microelectrodes can be fabricated on one small chip for high-throughput fusion. Transparent chip architecture benefited for microscopic observation and real-time recording. The integration of the experimental platform, signal generator and microchip is helpful for developing a portable cell-fusion system. On-chip electrofusion experiments of cucumber mesophyll protoplasts have been carried out and up to 40% fusion efficiency was obtained.

Info:

Periodical:

Edited by:

Zhou Mark

Pages:

664-667

DOI:

10.4028/www.scientific.net/AMM.52-54.664

Citation:

J. Yang et al., "Design and Performance of a High-Efficiency Cell-Electrofusion Device", Applied Mechanics and Materials, Vols. 52-54, pp. 664-667, 2011

Online since:

March 2011

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Price:

$35.00

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