Research on Biochemical Materials with Hydroxylation of Androst-4-en-3,17-dione by Colletotrichum lini AS3. 4486

Abstract:

Microbial transformation of androst-4-en-3,17-dione (AD; 1) using Colletotrichum lini AS3. 4486 resulted in the production of two metabolites 2 and 3. The structures of these compounds were elucidated by spectroscopic analysis (LC-MS, FTIR and NMR) as 15α-hydroxyandrost-4-en-3,17-dione (15α-OH-AD; 2) and 11α,15α-dihydroxyandrost-4-en-3,17-dione (11α,15α-diOH-AD; 3). AD underwent regioselective hydroxylation at 15α position, subsequently hydroxylated at 11α position and converted to compound 3. 11α,15α-diOH-AD as an important metabolic product was pharmaceutical intermediate and the yield was up to 97.58% when the concentration of substrate was 4 g L^-1.

• Periodical: Applied Mechanics and Materials (Volume 540)
• Pages: 347-351
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.540.347
• Online since: April 2014
• Authors: Jia Jia Ren, Yan Bing Shen, Ri Le Ge, Min Wang*
• Keywords: Androst-4-en-3,17-dione, Biotransformation, Colletotrichum lini, Hydroxylation

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