Model Optimization and Stability of Hemoglobin Analysis in Human Soluble Blood Samples by FTIR/ATR Spectroscopy

Tao Pan; Ai Hong Peng; Wen Jie Huang

doi:10.4028/www.scientific.net/AMM.55-57.1168

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 55-57Model Optimization and Stability of Hemoglobin...

Model Optimization and Stability of Hemoglobin Analysis in Human Soluble Blood Samples by FTIR/ATR Spectroscopy

Abstract:

Using Fourier transform infrared spectroscopy (FTIR), attenuated total reflection (ATR) technology and partial least square (PLS) method, the rapid quantification method of hemoglobin (HGB) in human soluble blood samples was established. Based on the distribution of samples’ HGB chemical value and absorbance on 1543 cm^-1 which had the highest signal to noise ratio for HGB, all samples were divided into calibration set and prediction set for 50 times. PLS models were established for all divisions, based on the average data RMSEP_Ave, the stable optimal model was selected, the corresponding PLS factor, RMSEP_Ave and R_P,Ave were 2, 6.81 g/L and 0.943 respectively.

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Periodical:

Applied Mechanics and Materials (Volumes 55-57)

Pages:

1168-1171

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.55-57.1168

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Online since:

May 2011

Authors:

Tao Pan, Ai Hong Peng, Wen Jie Huang

Keywords:

ATR, FT-IR, Hemoglobin, Human Soluble Blood Sample, PLS, Stability

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