Survival of Pseudomonas putida for Biodiesel Blend (B5) in Soil Bioremediation

Abstract:

The soil-contaminated areas can be cleaned-up by the emerging science and technology of bioremediation. This study aims to investigate the survival of Pseudomonas putida (P. putida) as biodegradable agent in soil contaminated with biodiesel/diesel blends (B5). The spillage simulation of B5 was conducted at laboratory scale for 24 days of treatment. The results show that contamination of biodiesel/diesel blends in soil induced a reduction growth of P. putida. The growth of P. putida was measured high in control compared to B5 sample proved the inhibitory effect has occurred. Nevertheless, the P. putida colonies counts for sample B5 were persisted high until the end of treatment period at 5.0 x10⁶ CFU/ml. P. putida is very sensitive to any ecosystem perturbation therefore this study notably serves as necessary information and potentially exploits as a useful oil-soil biodegradable agent in soil bioremediation.

• Periodical: Applied Mechanics and Materials (Volume 567)
• Pages: 139-143
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.567.139
• Online since: June 2014
• Authors: Norshuhaila Mohamed Sunar*, Quin Emparan, Ahmad Tarmizi Abdul Karim, S.F.M. Noor, M. Maslan
• Keywords: Biodiesel Blend (B5), Bioremediation, P. putida

References

[1] M. Farhadian, C. Vachelord, D. Duchez, C. Larrochec, In situ bioremediation of monoaromatic pollutants in groundwater : a review, Bioresource Techn. 99 (2008) 5296-5308.

DOI: 10.1016/j.biortech.2007.10.025

[2] Information on http: /epp. eurostat. cec. eu. int.

[3] Information on http: /www. biodiesel. org/docs/ffs-basics/clean-cities-fact-sheet-biodiesel-blends-april. pdf?sfvrsn=4.

[4] N. M. Sunar, Q. Emparan, A. T. A. Karim, S. F. M. Noor, M. Maslan, F. Mustafa, N. Khaled, The effectiveness of Pseudomonas putida 49128 as biodegradable agent in biodiesel soil contamination in Proc. INCIEC, Springer Singapore, (Part VIII), (2014).

DOI: 10.1007/978-981-4585-02-6_70

[5] T. Watanabe, T. Hiramaya, Genotoxicty of Soil, J. Health Sci. 47 (2001) 433-438.

[6] A.P. Mariano, R.C. Tomasella, D.L.M. Oliveira, J.C., D. Angelis, Biodegradability of diesel and biodiesel blends, African Journal of Biotechnology, 7 (2008) 1323-1328.

[7] S.Y. Wang, C. Vipulanandan, Biodegradation of naphthalene contaminated soils in slurry bioreactor, J. Environ. Eng. 127 (2001) 748-754.

DOI: 10.1061/(asce)0733-9372(2001)127:8(748)

[8] N. M. Sunar, Q. Emparan, A. T. A. Karim, S. F. M. Noor, M. Maslan, F. Mustafa, N. Khaled, The effectiveness of bioremediation treatment for diesel-soil contamination, Advanced Materials Research, 845 (2014) 817-823.

DOI: 10.4028/www.scientific.net/amr.845.146

[9] N. M. Sunar, Q. Emparan, A. T. A. Karim, S. F. M. Noor, M. Maslan, F. Mustafa, N. Khaled, Bioremediation of bio-fuel-soil contamination by using Pseudomonas putida, Advanced Materials Research, 845 (2014) 138-145.

DOI: 10.4028/www.scientific.net/amr.845.138

[10] L. T, Taylor, and D.M. Jones, Bioremediation of coal tar PAH in soil using biodiesel, Chemosphere. 44 (2001) 1131-1136.

DOI: 10.1016/s0045-6535(00)00344-1

[11] A. Mrozik, B. Piotrowska-Seget, S. Labuzek, Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons, Polish J. Environ. Stud. 12 (2003) 15-25.

[12] T. Schleicher, R. Werkmeister, W. Russ, R. M. Piroff, Microbiological stability of biodiesel-diesel mixture, Bioresour. Technol. 100 (2009) 724-730.

DOI: 10.1016/j.biortech.2008.07.029