Development and Application of Molecular Biology Techniques in Environmental Treatment

Article Preview

Abstract:

The application of molecular biology technique to expand the breadth and depth of the study of environmental microbiology, the researchers from the microscopic point of view more detailed deep understanding of the physiological and biochemical mechanisms of microorganisms on the degradation of pollutants, and at the same time, the advanced molecular biology technology for environmental monitoring, environmental pollution treatment, bioremediation of the contaminated environment provides the basis and method for faster, more sensitive, more scientific, which greatly promoted the progress of pollution control practice. The basic theory of molecular biology technology and its application in wastewater through the detection of microorganisms are shown.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

892-895

Citation:

Online since:

July 2014

Authors:

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] R Fani, G Damiani, C Di Serio, et al. 1993. Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for microorganisms. Mol Ecol, 2(4): 243~250.

DOI: 10.1111/j.1365-294x.1993.tb00014.x

Google Scholar

[2] C Guo, W Sun, JB Harsh, et al. 1997. Hybridization analysis of microbial DNA from fuel oil-contaminated and noncontaminated soil. Microbiol Ecol, 34(3): 178~187.

DOI: 10.1007/s002489900047

Google Scholar

[3] GS Sayer, MS Sheilds, ET Tedford. 1985. Application of DNA-DNA colony hybridization to the detection of catabolic genotypes in environmental samples. Appl EnviroMicrobiol, 49: 1295~1303.

DOI: 10.1128/aem.49.5.1295-1303.1985

Google Scholar

[4] S Selvaratnam, BA Schoedel, BL Mefarland, et al. 1995. Applica-tion of reverse transcriptase PCR for monitoring expression of catabolicdmpNgene in a phenol-degrading sequencing batch reactor. Appl Environ Microbiol, 61(11): 3981~3985.

DOI: 10.1128/aem.61.11.3981-3985.1995

Google Scholar

[5] EM Top, WE Holben, LJ Forney. 1995. Characterization of diverse 2, 4-dichlorophenoxyacetic acid degradative plasmids isolated from soil by complementation. Appl Environ Microbiol, 61(5): 11691~11698.

DOI: 10.1128/aem.61.5.1691-1698.1995

Google Scholar

[6] JB Stock, AM Stock, JM Mottonen. 1990. Signal transduction in bacteria. Nature, 344(6265).

DOI: 10.1038/344395a0

Google Scholar

[7] WR Streber, KN Timmis, MH Zenk. 1987. Analysis, cloning, and high-level expression of 2, 4-dichloro phenoxyaceate monooxygenase genetfdAof Alcaligenes eutrophus JMP134. JBacteriol, 169(7): 2950~2955.

DOI: 10.1128/jb.169.7.2950-2955.1987

Google Scholar

[8] J, Borneman RJ Hartin. 2000. PCR primers that amplify fungal rRNA genes from environmental samples. Appl Environ Microbiol, 66(10): 4356~4360.

DOI: 10.1128/aem.66.10.4356-4360.2000

Google Scholar

[9] E Carr, H Eason, S Feng. 2001. RAPD-PCR typing of Acinetobacter isolates from activated sludge systems designed to remove phosphorus. Microbiology, 90: 309~319.

DOI: 10.1046/j.1365-2672.2001.01245.x

Google Scholar

[10] G Siyn-Robets, G Lewis. 2001 In situ analysis of Nitrosomonas spp. in wastewater treament wetland biofilms[J]. Wat Res, 11(35): 2731—2739.

DOI: 10.1016/s0043-1354(00)00544-3

Google Scholar

[11] P Yongzhen, G Shouyou. The applications of molecular biogical tools to microbial ecosystem analyses in wastewater treatment process[J]. Acta Scientiae Circumastantiae, 2005, 25(9): 1143-1147.

Google Scholar