Laccase Incorporated into PEG-PLA Polymer as Active and Stable Biocatalyst for Ionic Liquids Media

Muhammad Moniruzzaman; Yoichiro Nao; Sekhar Bhattacharjee; Tsutomu Ono

doi:10.4028/www.scientific.net/AMM.625.333

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 625Laccase Incorporated into PEG-PLA Polymer as...

Laccase Incorporated into PEG-PLA Polymer as Active and Stable Biocatalyst for Ionic Liquids Media

Abstract:

Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, M_W = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) [C₂mim][PF₆] (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330-480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions.

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Periodical:

Applied Mechanics and Materials (Volume 625)

Pages:

333-336

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.625.333

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Online since:

September 2014

Authors:

Muhammad Moniruzzaman*, Yoichiro Nao, Sekhar Bhattacharjee, Tsutomu Ono

Keywords:

Enzyme Activity, Enzyme Immobilization, Enzyme Stability, Ionic Liquid (IL), Laccase Poly (Ethylene Glycol)-Block-Polylactide Emulsions

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