Phase Diagram of an Aqueous Salt-Polymer System Composed of Poly Ethylene Glycol 4000 + Na3C6H5O7+ H2O

Article Preview

Abstract:

Liquid−liquid extraction for the poly ethylene glycol (PEG) + Na3C6H5O7 + H2O system, with PEG of molecular weight 4000, have been measured at 300.15 K. Phase diagram behavior and tielines for PEG4000 have been studied and the effects of molecular weight of PEG 2000,4000,6000 on the binodal curve was investigated. In comparing to literature it was found that an increase in molecular weight of PEG shifted the binodal curve to lower PEG and salt concentrations. The ATPS's studied may be used in separation of protein and other compounds.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

574-577

Citation:

Online since:

September 2014

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] Raghavarao, K., et al., Aqueous two-phase extraction for downstream processing of enzymes/proteins. Advances in applied Microbiology, 1995. 41: pp.97-171.

DOI: 10.1016/s0065-2164(08)70309-5

Google Scholar

[2] Johansson, G. and H. Walter, Partitioning and concentrating biomaterials in aqueous phase systems. International review of cytology, 1999. 192: pp.33-60.

DOI: 10.1016/s0074-7696(08)60521-5

Google Scholar

[3] Asenjo, J.A., Separation processes in Biotechnology. Vol. 9. 1990: CRC.

Google Scholar

[4] Huddleston, J.G., H.D. Willauer, and R.D. Rogers, Phase diagram data for several PEG+ salt aqueous biphasic systems at 25 C. Journal of Chemical & Engineering Data, 2003. 48(5): pp.1230-1236.

DOI: 10.1021/je034042p

Google Scholar

[5] Murugesan, T. and M. Perumalsamy, Liquid-liquid equilibria of poly (ethylene glycol) 2000+ sodium citrate+ water at (25, 30, 35, 40, and 45) C. Journal of Chemical & Engineering Data, 2005. 50(4): pp.1392-1395.

DOI: 10.1021/je050081k

Google Scholar

[6] Amaresh, S.P., et al., Liquid− Liquid Equilibrium of Poly (ethylene glycol) 4000+ Diammonium Hydrogen Phosphate+ Water at Different Temperatures. Journal of Chemical & Engineering Data, 2008. 53(7): pp.1574-1578.

DOI: 10.1021/je800118c

Google Scholar

[7] Regupathi, I., et al., Liquid− Liquid Equilibrium of Poly (ethylene glycol) 6000+ Triammonium Citrate+ Water Systems at Different Temperatures. Journal of Chemical & Engineering Data, 2009. 54(3): pp.1094-1097.

DOI: 10.1021/je8008478

Google Scholar

[8] Zafarani-Moattar, M.T., R. Sadeghi, and A.A. Hamidi, Liquidâ€"liquid equilibria of an aqueous two-phase system containing polyethylene glycol and sodium citrate: experiment and correlation. Fluid phase equilibria, 2004. 219(2): pp.149-155.

DOI: 10.1016/j.fluid.2004.01.028

Google Scholar

[9] Sivars, U. and F. Tjerneld, Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins1. Biochimica et Biophysica Acta-General Subjects, 2000. 1474(2): pp.133-146.

DOI: 10.1016/s0304-4165(99)00208-1

Google Scholar