Construction of cDNA Library of Hansenula anomala

Abstract:

To investigate the molecular mechanism of ester-producing of acetate yeast, a cDNA library was constructed based on Switching Mechanism At 5’ end of the RNA Transcript (SMART) technique using Construction Kit. The result of the test showed that the primary constructed cDNA library had a cloning capacity of 3×10⁶ and the length of the inserted fragment was between 1-3kb,the coverage of cDNA library was 74.07%. The quality of the library could meet the requirements.

• Periodical: Applied Mechanics and Materials (Volume 665)
• Pages: 363-366
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.665.363
• Online since: October 2014
• Authors: Shang Ling Fang, Jin Yan, Mao Bin Chen, Dong Sheng Xue, Jing Hua Cao*
• Keywords: cDNA Library, Hansenula anomala, Smart

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