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Study on ELISA Plates Modified with Methylacrylic Acid by 60Co Irradiation

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Abstract:

Surface modified ELISA plates grafted with methylacrylic acid (MAA) were prepared by 60Co γ-Ray irradiation at room temperature in MAA water solution. The optimum conditions of modification were decided by the water contact angle and the absorbency of polystyrene enzyme label plate. FTIR, XRD spectra and AFM images show that MAA and its polymer have been grafted to the surface of the ELISA plates induced by 60Co γ-Ray irradiation. The volume concentration of MAA aqueous solutions reached 10%, and the radiative intensity was 10kGy, and correspondingly, the content of carboxyl group on the surface of ELISA plates reached 0.30mmol/cm2.

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Periodical:

Applied Mechanics and Materials (Volumes 670-671)

Pages:

219-223

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.670-671.219

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Online since:

October 2014

Authors:

Di Chen, Hua Shan Wang*

Keywords:

ELISA Plates, Irradiation, Methylacrylic Acid, Surface Modification

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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