Isolation, Structure Identification and Cytotoxicity Evaluation of Three Steroids from Boletus edulis

Abstract:

BEL-7402, HT-29, SPC-A1, U-251 bioassay-guided fractionation led to the isolation of the three active constituents from a wild fungi, Boletus edulis Bull. Ex Fr., which collected from Changbai Montain area. These three compounds’ chemical structures were elucidated on the base of spectroscopic data and comparison with known compounds as 5-cholestene-2,3-oxide (1), β-sitosterol (2), stigmasterol (3). All of the three compounds were extracted from Boletus edulis for the first time, and compound 1 was significantly cytotoxic against the four human cancer cell lines (BEL-7402, HT-29, SPC-A1 and U-251) with IC50 values 6.29, 6.98, 4.79 and 7.54 μM, respectively. Results of the study could supply referencs to the people to find the new drug from the fungus.

• Periodical: Applied Mechanics and Materials (Volumes 675-677)
• Pages: 1670-1673
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.675-677.1670
• Online since: October 2014
• Authors: Qi Lan Liang
• Keywords: Boletus edulis, Cytotoxicity, Structure Elucidation

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