Optimization of Fermentation Conditions for Cellulase Production by HDZK-BYTF620 (Aureobasidium pullulans) which is from the Gut of Termites

Abstract:

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl₂, MgSO₄ and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl₂, MgSO₄ and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10^-3 IU/mL, increased 5.0 % to that of using initial medium.

• Periodical: Applied Mechanics and Materials (Volume 692)
• Pages: 167-171
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.692.167
• Online since: November 2014
• Authors: Ming Jing Sun, Li Qing Sun, Shan Shan Wang, Kai Zhao*
• Keywords: Cellulase Producing Bacterium, Fermentation Culture Condition, Response Surface, Termite

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