Thin-Shell Silk Cocoon (TSC) as a Nitrogen Source of ABE Fermentation by Clostridium acetobutylicum

Natshera Kittithanesuan; Muenduen Phisalaphong

doi:10.4028/www.scientific.net/AMM.705.14

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 705Thin-Shell Silk Cocoon (TSC) as a Nitrogen Source...

Thin-Shell Silk Cocoon (TSC) as a Nitrogen Source of ABE Fermentation by Clostridium acetobutylicum

Abstract:

A thin-shell silk cocoon (TSC), a residual from the silk industry, was used as an alternative nitrogen (N-) source in ABE fermentation by Clostridium acetobutyricum ATCC 824. The experimental studies were performed in batch fermentation at 35 °C and pH 5.0. The results were compared with the system using yeast extract (YE) as N-source. It was shown that TSC fragments (TSC_f) could be used as a cheap substitute for YE for a certain amount. Under optimum conditions, total solvent of 22.3 g/L (12.7 g/L butanol, 8.0 g/L acetone and 1.5 g/L ethanol) was obtained from the ABE fermentation using the mixture of TSC_fand YE as N-sources. The ABE productivity and conversion yield were 0.15 g/L/h and 0.30 g/g sugar consumption, respectively.

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Periodical:

Applied Mechanics and Materials (Volume 705)

Pages:

14-18

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.705.14

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Online since:

December 2014

Authors:

Natshera Kittithanesuan, Muenduen Phisalaphong*

Keywords:

ABE Fermentation, Clostridium acetobutylicum, Silk Cocoon, Sugar Cane Juice

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* - Corresponding Author

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