A PCR Chip with Reusable Electrodes and its Temperature Analysis

Jing Tong Cao; Feng Cui; Wei Chen; Zhao Xin Guo; Wen Yuan Chen; Xiao Sheng Wu; Wu Liu; Wei Ping Zhang

doi:10.4028/www.scientific.net/AMM.742.54

Paper Titles

Study on Temperature’s Character of Eddy Current Sensor
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The Research of Maglev Gyroscope Data Characteristics Based on Two Positioning
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Refractive-Index Sensor Based on Metal-Insulator-Metal Waveguides
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The Study on After-Treatment System and Algorithm of Piezoelectric Micromachined Modal Gyroscope
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A PCR Chip with Reusable Electrodes and its Temperature Analysis
p.54

Evaluation of Measurement Uncertainty for Ball Pressure Test Based on Reading Microscope
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Measurement Uncertainty Sources Analysis and Verification of Time-Grating with Variable Coupling Coefficient
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Quality Evaluation Apparatus and Method for Laser Shock Processing the Weld Used in FDSO
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Research on Mass Determination of International Comparison CCM.M-K4 of 1 kg Mass Weights
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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 742A PCR Chip with Reusable Electrodes and its...

A PCR Chip with Reusable Electrodes and its Temperature Analysis

Abstract:

This paper presents a new micromachined PCR chip with separable electrodes part and reaction chamber part. The electrodes part, employing Pt film resistor as heaters and sensors, is reusable, and the chamber part is a disposable PDMS-GLASS bonded structure. This PCR chip with reusable electrodes can largely reduce fabrication cost. COMSOL software is used to simulate the temperature distribution of the designed chip and then use infrared thermometer (NEC R300SR) to verify temperature distribution of the fabricated chip. The test results turned out to perfectly fit the simulation of temperature distribution, which provides reliable basis for further PCR amplification experiment.