The Freeze-Dry Method and In Vitro Assay of Chitosan/Gelatin/Hydroxyapatite Artificial Bone Scaffolds

Abstract:

This study aims to create avirulent artificial bone scaffolds. Chitosan/gelatin mixture is blended with hydroxyapatite (HA) powder, followed by being processed with a free-dry method in order to form CGH artificial bone scaffolds. A stereomicroscope, an optical microscope and an MTT assay are used to evaluate the applications of the bone scaffolds. The combination of HA powders leads to isotropic pores in the bone scaffolds, while not inflicting their biocompatibility. In addition, the cell viability increases with the increasing content of HA powder. This study successfully produces biocompatible and non-toxic bone scaffolds.

• Periodical: Applied Mechanics and Materials (Volume 749)
• Pages: 441-444
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.749.441
• Online since: April 2015
• Authors: Ching Wen Lou, Shih Peng Wen*, Wen Cheng Chen, Yueh Sheng Chen, Jia Horng Lin
• Keywords: Artificial Bone Scaffolds, Avirulent, Biocompatibility, Freeze-Dry Method, Hydroxyapatite (HA)

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