Isolation of Potential Alkaliphilic Cellulase-Producer Strains from Limestone Area of Perlis

Nor Izzah Zainuddin; Khadijah Hanim Abd Rahman; Abdul Razak Shaari; Siti Jamilah Hanim Mohd Yusof

doi:10.4028/www.scientific.net/AMM.754-755.1054

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 754-755Isolation of Potential Alkaliphilic...

Isolation of Potential Alkaliphilic Cellulase-Producer Strains from Limestone Area of Perlis

Abstract:

Alkaliphilic cellulases-producer fungi were isolated from soil of limestone areas in Perlis. The potential strain was isolated by soil dilution plate method on enriched selective medium using CMC as substrate at different pH levels. Eleven isolates of different morphological colonies were screened using the hydrolysis capacity test by Gram’s iodine. Out of eleven colonies, five showed positive results as the hydrolysis zone formed. BK1 showed the highest hydrolysis capacity among all sample strains for every pH levels. Eventually, four strains were selected to be further explored as cellulolytic fungi for the production of alkaline cellulase in the future.

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Periodical:

Applied Mechanics and Materials (Volumes 754-755)

Pages:

1054-1058

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.754-755.1054

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Online since:

April 2015

Authors:

Nor Izzah Zainuddin*, Khadijah Hanim Abd Rahman, Abdul Razak Shaari, Siti Jamilah Hanim Mohd Yusof

Keywords:

Alkaliphilic Cellulases, Lignocellulose, Lignocellulosic Biomass, Pretreatment

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References

[1] J. Perez, J. Munoz-Dorado, T. De-La Rubia and J. Martinez: Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview. Int Microbiol. 5 (2002), 53-63.

DOI: 10.1007/s10123-002-0062-3

Google Scholar

[2] H.T. Tan, & K.T. Lee: Understanding the impact of ionic liquid pretreatment on biomass and enzymatic hydrolysis. J. Chem. Eng., 183 (2012) 448-458.

DOI: 10.1016/j.cej.2011.12.086

Google Scholar

[3] Z. Wang, D.R. Keshwani, A.P. Redding, J.J. Cheng, & J. Jay: Sodium hydroxide pretreatment and enzymatic hydrolysis of coastal Bermuda grass. Biores. Technol., 101 (2010) 3583-3585.

DOI: 10.1016/j.biortech.2009.12.097

Google Scholar

[4] J.Y. Park, R. Shiroma, M.I. Al-Haq: A novel lime pretreatment for subsequent bioethanol production from rice straw-calcium capturing by carbonation (CaCCO) process. Biores. Technol., 101(2010) 6805-6811.

DOI: 10.1016/j.biortech.2010.03.098

Google Scholar

[5] M. Pedersen, K. S. Johansen and A. S. Meyer: Low temperature lignocellulose pretreatment: Effects and interactions of pretreatment pH are critical for maximizing enzymatic monosaccharide yields from wheat straw. Biotechnol. Biofuels, 4: 11(2011).

DOI: 10.1186/1754-6834-4-11

Google Scholar

[6] G. Immanuel, R. Dhanusha, P. Prema, A. Palavesam: Effect of different growth parameters on endoglucanase enzyme activity by bacteria isolated from coir retting effluents of estuarine environment. Int J Env Sci Technol., 3 (2006), 25-34.

DOI: 10.1007/bf03325904

Google Scholar

[7] R.C. Kasana, R. Salwan, H. Dhar, S. Dutt, & A. Gulati: A rapid and easy method for the detection of microbial cellulases on agar plates using Gram's iodine. Current Microbial., 57 (2008), 503-507.

DOI: 10.1007/s00284-008-9276-8

Google Scholar

[8] K. Vega, G.K. Villena, V.H. Sarmiento, Y. Ludeña, N. Vera, & M. Gutiérrez-Correa: Production of alkaline cellulase by fungi isolated from an undisturbed rain forest of Peru. Biotechnol. Res. Int., (2012).

DOI: 10.1155/2012/934325

Google Scholar

[9] R. P., Gupta, P. Gigas, H. Mohapatra, V.K. Goswani and B. Chauhan: Microbial α-amylase; A biotechnological perspective. Process Biochem., 38 (2003), 1599-1616.

DOI: 10.1016/s0032-9592(03)00053-0

Google Scholar