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Investigation of the Effect of Ultrasound on Cell Growth

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Abstract:

Several studies have been made on cell growth by the ultrasound irradiation. It is important to change the output, irradiation time and duty cycle for cell growth. The duty cycle determined the proportion of the time that the ultrasound was “on”. We can control cell growth by changing these three terms. The purpose of this study is to find the optimum conditions for cell growth and examine a new factor to influence cell growth. Cell concentration was determined by measuring the absorbance with a spectrophotometer. In this study, the absorbance became highest on 10min ultrasound irradiation and the duty cycle of 67% in experimental conditions. However, when 30 min ultrasound irradiation and the duty cycles of 86%, the absorbance became close to the highest value too. The results suggested that, in addition to the duty cycle, the off time of ultrasound might also affect the cell growth.

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Periodical:

Applied Mechanics and Materials (Volume 870)

Pages:

447-452

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.870.447

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Online since:

September 2017

Authors:

Yusuke Tamai, Kozo Taguchi*

Keywords:

Absorbance, Duty Cycle, Off Time, Ultrasound, Yeast Cells

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© 2017 Trans Tech Publications Ltd. All Rights Reserved

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