Encapsulation of Watermelon Rind Extract in Chitosan Microcapsules: Analysis of Physical Properties and Flavonoid Release Ability

Abstract:

Along with the increase in watermelon production, the amount of watermelon rind waste increased. The total mass of fruit rind in a watermelon reaches around 30 percent and this fruit rind can increase the quantity of organic waste in Indonesia. The outer portion of the watermelon rind has a green layer containing a large amount of anthocyanin and a white layer containing flavonoids. In this study, the extract of watermelon rind containing anthocyanins and flavonoids was protected from damaging conditions using the ionic gelation encapsulation method. Chitosan (CN) was used as a natural polymer in this encapsulation method and sodium tripolyphosphate (TPP) was used as an ionic crosslinking agent. The total of flavonols content (TF), microstructure test, in vitro releasing test, and shelf life of microcapsules were observed in the various ratio between watermelon rind and the solvent. From the process, can be concluded that higher watermelon skin levels will produce the most flavonoid microcapsules (70g/35 mL). At 70g/35 mL or 2:1 g/mL watermelon skin levels give the best flavonoid release test results, especially if it will be applied to the pharmaceutical industry, which follows a controlled release method.

• Periodical: Applied Mechanics and Materials (Volume 908)
• Pages: 113-118
• DOI: https://doi.org/10.4028/p-sfup70
• Online since: August 2022
• Authors: Venitalitya A.S. Augustia*, Kamariah Kamariah, Fitri Mulia, Dewanti Siwi Nurani
• Keywords: Chitosan, Encapsulation, Flavonoid Release, Physical Properties, Red Watermelon Rind

References

[1] Anonim, 2015. Statistik Produksi Hortikultura Tahun 2014, Jakarta: Direktorat Jenderal Hortikultura, Kementerian Pertanian.

[2] Andrianto, F., 2016. Pengaruh Sari Kulit dan Buah Semangka Merah (Citrullus lanatus) Sebagai Bahan Pengencer Terhadap Motilitas dan Viabilitas Spermatozoa Domba, Surabaya: Fakultas Kedokteran Hewan, Universitas Airlangga.

DOI: 10.15395/mkb.v48n3.842

[3] S. Mallek-Ayadi, N. Bahloul, and N. Kechaou, Characterization, phenolic compounds and functional properties of Cucumis melo L. peels,, Food Chem., vol. 221, p.1691–1697, (2017).

DOI: 10.1016/j.foodchem.2016.10.117

[4] S. J. Maleki, J. F. Crespo, and B. Cabanillas, Anti-inflammatory effects of flavonoids,, Food Chem., vol. 299, no. July, (2019).

[5] McDougall G J, Fyffe S, Dobson P, Stewart D. 2007. Anthocyanins from red cabbage-stability to simulated gastrointestinal digestion. Phytochemistry, 68, 1285–1294.

DOI: 10.1016/j.phytochem.2007.02.004

[6] Sunggil K, Doohyun B. Dong Y C, Eul-Tai L, Moo-Kyoung Y. 2009. Identification of two novel inactive DFR-A alleles responsible for failure to produce anthocyanin and development of a simple PCR-based molecular marker for bulb color selection in onion (Allium cepa L.). Theoretical and Applied Genetics, 118, 1391–1399.

DOI: 10.1007/s00122-009-0989-2

[7] Wang J, Mazza G. 2002. Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor alpha in LPS/IFN-gamma-activated RAW 264.7 macrophages. Journal of Agricultural and Food Chemistry, 50, 4183–4189.

DOI: 10.1021/jf011613d

[8] Hou D X. 2003. Potential mechanisms of cancer chemoprevention by anthocyanins. Current Molecular Medicine, 3, 149–159.

DOI: 10.2174/1566524033361555

[9] Kong L, Chia N, Goh T, Chia R. 2003. Brouillard, analysis and biological activities of anthocyanins. Phytochemistry, 62, 923–933.

DOI: 10.1016/s0031-9422(03)00438-2

[10] M. Shafiq and Z. Singh, Pre-harvest spray application of phenylpropanoids influences accumulation of anthocyanin and flavonoids in 'Cripps Pink' apple skin,, Sci. Hortic. (Amsterdam)., vol. 233, no. February, p.141–148, (2018).

DOI: 10.1016/j.scienta.2018.01.020

[11] S. C. S. R. De Moura, C. L. Berling, S. P. M. Germer, I. D. Alvim, and M. D. Hubinger, Encapsulating anthocyanins from Hibiscus sabdari ff a L . calyces by ionic gelation : Pigment stability during storage of microparticles,, Food Chem., vol. 241, no. August 2017, p.317–327, (2018).

DOI: 10.1016/j.foodchem.2017.08.095

[12] Jamekhorshid, A., Sadrameli, S.M., Farid, M., 2014. A review of microencapsulation methods of phase change materials (PCMs) as a thermal energy storage (TES) medium. Renew. Sust. Energ. Rev. 31, 531-542.

DOI: 10.1016/j.rser.2013.12.033

[13] Gharsallaoui, A., Roudaut, G., Chambin, O., Voilley, A., Saurel, R., 2007. Applications of spray-drying in microencapsulation of food ingredients: An overview. Food Res. Int. 40, 1107-1121.

DOI: 10.1016/j.foodres.2007.07.004

[14] Gou, G.J., Dong, L.E., Bao, F.J., Wang, Z.Y., Jiao, L., Huang, J., Sun, Y., Xue, B., 2013. A review on research of the sustained release drug delivery system based on magnesium aluminate layered double hydroxide. Appl. Mech. Mater. 320, 495-504.

DOI: 10.4028/www.scientific.net/amm.320.495

[15] L. E. Kurozawa and M. D. Hubinger, Hydrophilic food compounds encapsulation by ionic gelation,, Curr. Opin. Food Sci., vol. 15, p.50–55, (2017).

DOI: 10.1016/j.cofs.2017.06.004

[16] Tsai, M.-L., Chen, R.-H., Bai, S.-W., & Chen, W.-Y. (2011). The storage stability of chitosan/tripolyphosphate nanoparticles in a phosphate buffer. Carbohydrate Polymers, 84(2), 756–761.

DOI: 10.1016/j.carbpol.2010.04.040

[17] Long L, Scardino A, Vasapollo G. 2007. Identification and quantification of anthocyanins in the berries of Pistacialen tiscus L., Phillyreala tifolia L. and Rubia peregrine L. Innovative Food Science and Emerging Technologies, 8, 360–364.

DOI: 10.1016/j.ifset.2007.03.010

[18] Domaratzki, R. E., & Ghanem, A. (2013). Encapsulation and release of cladribine from chitosan nanoparticles? Journal of Applied Polymer Science, 128(3), 2173–2179.

DOI: 10.1002/app.38354

[19] Rodrigues A S, Pérez M R, García M S, Simal J, Almeida D P. 2011. Effect of meteorological conditions on antioxidant flavonoids in portuguese cultivars of white and red onions. Food Chemistry, 124, 303–308.

DOI: 10.1016/j.foodchem.2010.06.037

[20] Pérez M R, Regueiro J, Simal J, Rodrigues A S, Almeida D P. 2014. Increasing the added-value of onions as a source of antioxidant flavonoids: A critical review. Critical Reviews in Food Science & Nutrition, 54, 1050–1062.

DOI: 10.1080/10408398.2011.624283

[21] Sarabandi, K., Jafari, S. M., Mahoonak, A. S., & Mohammadi, A. (2019). Application of gum Arabic and maltodextrin for encapsulation of eggplant peel extract as a natural antioxidant and color source. International Journal of Biological Macromolecules, 140, 59–68.

DOI: 10.1016/j.ijbiomac.2019.08.133