Optimization of Conditions for Extracting Muscle Protein from Grass Carp Using Response Surface Methodology

Fu Lan Zhang; Yong Le Liu; Jian Yu; Qiang Li; Xiang Hong Li; Jian Hui Wang; Fa Xiang Wang

doi:10.4028/www.scientific.net/AMR.1004-1005.837

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1004-1005Optimization of Conditions for Extracting Muscle...

Optimization of Conditions for Extracting Muscle Protein from Grass Carp Using Response Surface Methodology

Abstract:

Protein is the major component of muscle tissue, in order to analyze change rule of the grass carp muscle protein during storage, the Plackett–Burman design and response surface methodology were employed to optimize its extraction conditions. The results showed that pH, SDS concentration and liquid-solid ratio were the significant factors influencing response, and the final combination of the optimized parameters was generated: pH, 8.3; liquid-solid ratio, 17:1; SDS concentration, 3.50%;beta mercaptoethanol concentration,0.3%; extraction temperature, 25 °C; extraction time, 1 h; ultrasonic time,15 min. And under these conditions, the protein extraction rate increased to 92.42%.

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Periodical:

Advanced Materials Research (Volumes 1004-1005)

Pages:

837-840

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1004-1005.837

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Online since:

August 2014

Authors:

Fu Lan Zhang, Yong Le Liu, Jian Yu, Qiang Li, Xiang Hong Li, Jian Hui Wang, Fa Xiang Wang*

Keywords:

Extraction, Grass Carp, Muscle Protein, Plackett-Burman Design, Response Surface Methodology (RSM)

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* - Corresponding Author

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