Influence of Dihydromyricetin on Lowering Blood Glucose Concentration and Reducing Early Kidney Damage in Imparied Glucose Tolerance Rats

Abstract:

Diabetic nephropathy (DN) is a common complication of diabetes and it is related to irreversible kidney damages and chronic renal failure. Impaired glucose tolerance (IGT) is an early stage in the development of diabetes and DN. Early detection of IGT and treatment of its associated early kidney damage can effectively prevent the development of DN. In this paper, the influence of dihydromyricetin (DHM) on lowering blood glucose concentration and reducing early kidney damage in IGT rats was studied. Animal model of IGT rats was built by two week intragastric injection of D-galactose and treated with eight weeks of intragastric injection of DHM at two dose levels. The concentrations of fasting blood glucose (FBG), two-hour postprandial blood glucose (2hBG), insulin levels, contents of microalbuminuia (mAlb) and blood urea nitrogen (BUN), and activities of lactate dehydrogenase (LDH) in kidney were analysed and compared with those in control groups. Experimental results indicated that DHM treatment can significantly lower the levels of two-hour postprandial blood glucose and insulin, decrease the content of mAlb and the activities of LDH in kidney, but does not influence the level of BUN. The study suggested that DHM can effectively improve the states of IGT rats and provide a protective effect against early kidney damage.

• Periodical: Advanced Materials Research (Volumes 1004-1005)
• Pages: 857-863
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1004-1005.857
• Online since: August 2014
• Authors: Yong Qiang Zhou, Tao Yan Mao
• Keywords: Blood Urea Nitrogen, Dihydromyricetin, Early Kidney Damage, Lactose Dehydrogenase, Microalbuminuia

References

[1] E. Cadenas, K.J.A. Davies. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical. Biol. Med., 26, 222–230. (2000).

[2] C.L. Chen, Y.F. Wand and L. Ding. Comparison of two rapid methods to determine blood glucose with standard method. Journal of Nurses Training, 14(8), 12. (1999).

[3] Q. Du, W. Cai, M. Xia, et al. Purification of (+)-dihydromyricetin from leaves extract of ampelopsis grossedentata using high-speed countercurrent chromatograph with scale-up triple columns. J Chromatogr., A973, 217–220. (2002).

DOI: 10.1016/s0021-9673(02)01092-0

[4] L. Feng, On the relationship between essential modernisation and quality improvement of Chinese traditional medicine, Chinese J. Information Chinese Traditional Medicine (in Chinese), 10, 1-3. (2003).

[5] G. Franz, S. Alban, Structure-activity relationship of antithrombotic polysaccharide derivatives. Biol. Macromol., 17, 311–314. (1995).

DOI: 10.1016/0141-8130(96)81837-x

[6] Q. Gao, R. Seljelid, H. Chen, et al. Characterisation of acidic heteroglycans from tremella fuciformis berk with cytokine stimulating activity. Carbohydrate Research, 288, 135-142. (1996).

DOI: 10.1016/s0008-6215(96)90789-2

[7] C.J. Glueck, P. Wang, N. Goldenberg, et al. Pregnancy outcomes among women with polycystic ovary syndrome treated with metformin. Hum. Reprod., 17, 2858-2864. (2002).

DOI: 10.1093/humrep/17.11.2858

[8] P. Guo, H. Wang, T. Gu, et al. The research on the effect of promoting blood circulation and notifying the kidney decoction on LDH isoenzymes in the kidney tissue of chronic renal failure rats. Chinese Journal of Experimental Traditional Medical Formulae (in Chinese), 1, 26-28. (2001).

[9] K. Hase, P. Basnet. Effect of hovenia dulcis on lipopolysaccharide-induced liver injury in chronic alcohol-fed rats. J. Trad. Med., 14, 28–33. (1997).

[10] X.T. Le, H.P. Yin, M. Wang, Preparation of low molecular weight polysaccharide of spirulina platensis and anti-tumor activity of its sulfates ester in vitro. Pharmaceutical Biotechnology, 2, 119~122. (2006).

[11] J. Liu, T. Zhou. Study on biology of vine tea. Traditional Chinese Medicine (in Chinese), 30, 459-463. (1999).

[12] S.V. Mezhzherin, O.P. Berezovskaia. Characters of amino acid substitution in LDH-A and LDH-B in the evolutionary line of vertebrates. Tsitol. Genet., 37, 24-29. (2003).

[13] W. Nie, Y. Zhang, J. Zhou, Study on pharmacology of tremella fuciformis. Pharmacology & Clinical Chinese Medicine, 4, 44-46. (2000).

[14] C. Janelle, J.C. Nisbet, J.M. Sturtevant, et al. Metformin and serious adverse effects. Med. J. Australia, 180, 53-54. (2004).

DOI: 10.5694/j.1326-5377.2004.tb05796.x

[15] H. Qi, Q. Zhang, T. Zhao, et al. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int. J. Biol. Macromol., 37, 195-199. (2005).

DOI: 10.1016/j.ijbiomac.2005.10.008

[16] K. Sakai, T. Yamane, Y. Saitoh, et al. Effect of water extracts of crude drugs in decreasing blood alcohol concentrations in rats, Chem Pharm. Bull., 35, 4597-4604. (1987).

DOI: 10.1248/cpb.35.4597

[17] Y. She, Applied Isoenzymology, 2nd ed. (in Chinese), Ulaanbaatar: Inner Mongolia People Press, 108-110. (2001).

[18] G. Slama, The potential of metformin for diabetes prevention. Diabetes Metab., 29, 104-111. (2003).

[19] K. Uchida, Role of reactive aldehyde in cardiovascular diseases. Free Radical. Biol. Med., 28, 1685–1696. (2002).

DOI: 10.1016/s0891-5849(00)00226-4

[20] UKPDS (UK Prospective Diabetes Study Group) Effect of intensive blood-glucose control with metformin on complications in overweight patients with type 2 diabetes (UKPDS 34), Lancet, 352, 854-865. (1998).

DOI: 10.1016/s0140-6736(98)07037-8

[21] Q. Wu, C. Zheng, Z.X. Ning, Study on extracting tremella fuciformis polysaccharides with microwave-assisted extraction. Food Science & Technology, 9, 109-111. (2006).

[22] Z. Xiao, Chemistry of traditional Chinese medicine, Shanghai: Shanghai Press of Science and Technology. (1997).

[23] S.M. Yoo, S. Mun, J.H. Kim, Recovery and pre-purification of (+)-dihydromyricetin from hovenia dulcis. Process Biochemistry, 41, 567-570. (2006).

DOI: 10.1016/j.procbio.2005.10.008

[24] Y. Zhang, Z. Ning, W. Yang, Vine tea science (in Chinese), Guangzhou: Guangdong Press of Sciebce and Technology. (2003).

[25] Y. Zhang, Z. Ning, M. Hu, Current research and applications of flavonoid dihydromyricetin. Chinese Traditional Patent Medicine (in Chinese), 24, 970-972. (2002).

[26] C. Zheng, L. Yang, J.H. Chen, Mechanism of microwave-enhanced extraction of dihydromyricetin from ampelopsis grossedntata. Journal of Chemical and Engineering (China), 57(5), 1198-1203. (2006).

[27] Z. Zhong, J. Tan, G. Zhou, Pharmacology study on ampelopsin from guangxi yao vine tea. J. Med. Pharm. Chinese Minorities (in Chinese), 4, 42-44. (1998).