Determination of Aflatoxin Contaminants in Four South Medicines by HPLC

Abstract:

A method was developed for the simultaneous determination of Afiatoxin ( AFB₁、AFB₂、AFG₁、AFG₂) in four south medicines is developed by Liquid chromatograPhy with fluoreseence detection after immunoaffinity column clean一up and Pre一cloumn derivatization.four south medicine samples were extraeted with methanol一water (70:30,v/v),The extraets were Purified by imunoaffinity columns and the toxins were separated by reversed phase HPLC, and quantified with fluorescenee detection after photoehemieal derivatization. The separation was perfored on a C₁₈ column.Recoveries of the different medicines spiked with Aflatoxin, The average recoveries ranged from 94.0 %~92.3 % by addition of avermectin standards at three different concentration levels of 10 μg/kg) to four south medicines as matrixes. The sensitivity, accuracy and precisiothod were able to meet the requirements for pesticide residue analysis.

• Periodical: Advanced Materials Research (Volumes 1004-1005)
• Pages: 928-931
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1004-1005.928
• Online since: August 2014
• Authors: Yu Bing Zha, Xue Fu Liu, Chun Liang Yang, Xiao Fang Wang, Ling Lin, Shao Dong Zeng, Zhen Zhang, Yong Hui Zhe
• Keywords: Aflatoxins, Four South Medicines, HPLC

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