Paper Titles
Antibiotic Nalidixic Acid Removal by Activated Carbon Prepared from Keratin Wastes
p.895
Changes of Microstructure and Viscosity of Enzymatic-Deamidated Rice Protein
p.900
Effect of Storage Conditions on Apparent Viscosity of Oleogel Developed by β-Sitosterol and Lecithin with Sunflower Oil
p.903
Research on Aerobic Granular Sludge Cultivated under Selective Pressure
p.908
Determination of Tetracyclines Residues in Aquatic Products by HPLC
p.914
Development of a Hydroxypropyl-β-Cyclodextrin Complex for Improving Water Solubility and Reducing Skin Sensitivity of Salicylic Acid
p.919
Study on Terpene Phenolic Compounds and its Health Effects
p.924
Determination of Aflatoxin Contaminants in Four South Medicines by HPLC
p.928
Moisture Absorption Properties and Kinetic Analysis of Three Different Kinds of Gauze
p.932

Determination of Tetracyclines Residues in Aquatic Products by HPLC

Article Preview

Abstract:

A sensitive method for simultaneously determining residues of oxytetracycline, tetracycline ,chlortetracycline and doxycycline in aquatic product was established by HPLC. Samples were extracted with Na2EDTA-Mcllvaine buffer solutions and centrifuged.The sample extracts were cleaned up by C18 solid-phase extraction cartridge and then were determined by UV detection at 355 nm.At fortification levels of 0.005~0.1 mg/kg, the average recoveries were of 75%~96% , The RSDs were of 1.02%~6.56%,Limit of detection was 0.002 mg /kg for oxytetracycline,tetracycline and doxycycline and 0.005 mg /kg for chlortetracycline.The sensitivity, accuracy and precision of this method were able to meet the requirements for pesticide residue analysis.

You might also be interested in these eBooks
Advanced Materials and Technologies View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 1004-1005)

Pages:

914-918

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1004-1005.914

Citation:

Cite this paper

Online since:

August 2014

Authors:

Ling Lin, Hong Bin Guo, Qi Li, Li Qiang Zhang, Li Li Liu, Chun Liang Yang

Keywords:

Aquatic Products, Chlortetracycline, Doxycycline, HPLC, Oxytetracycline, Tetracycline

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] S. D Ye, S.N. Gao, Health technology of veterinary drug residues in food, Tianjin Science and Technology Press, Tianjin, (1998).

Google Scholar

[2] Wei. Li, Zhen. Yan, Discussion of oxytetracycline, tetracycline, chlortetracycline residues determination method of animal meat,J. Chinese Journal of Health Laboratory, 11(2001) : 730-731.

Google Scholar

[3] L.M. Chi, Z. L. Quan X.M. Zhang, Determination of tetracycline antibiotics in pork meat by HPLC,J. Anhui Agricultural Sciences, 38(2010): 3548-3549.

Google Scholar

[4] Yan. Jin, Li. Jiang, Yan. Zhao, Yan. Lin, Determination of residues of tetracycline antibiotics by HPLC,J. Journal of Shenyang Normal University, 24(2006): 218-220.

Google Scholar

[5] G.F. Pang Y.Z. Cao J.J. Zhang G.Q. Jia C.L. Fan X.M. Li, Study on Simultaneous Determination of tetracycline, chlortetracycline, oxytetracycline and doxycycline residues in poultry meat by HPLC,J. Journal of instrumental analysis, 24(2005): 61-63.

Google Scholar

[6] B.L. Cai S.R. Xu, Cheng Qian C.L. Lu,S.J. Chen, Bin. Yan, determination of tetracycline antibiotics residues in meat and meat products for export by HPLC,J. 7(1997): 10-13.

Google Scholar

[7] CastellariM, Garcia-Regueiro JA. HPLC Determination of tetracyclines in lambmusele using an RP-C18 monclithic Type Column. Chromatographia,J. 58(2003): 789-792.

DOI: 10.1365/s10337-003-0068-9

Google Scholar

[8] Y.H. Liu C.P. Zhang L.Q. Men Z.H. Liu S.H. Wang, Determination of tetracyclines residues in chicken muscle by LC-MS,J. 24(2006): 171-173.

Google Scholar

[9] Wei. Jia, Lu. Sun X.G. Shi X.Y. Chen D.F. Zhong, Determination of 4 tetracyclines residues in milk by LC-MS, Journal of Shenyang Pharmaceutical University,J. 19(2002): 96-100.

Google Scholar

[10] H.F. Yang M.Y. Li, Wen. Chen, Food hygiene standard manual of physical and chemical testing, Chinese Standard Press, Beijing, 1997: 267-268.

Google Scholar

[11] S.Q. Zhu C.Q. Niu H.M. Zhang, Separation of tetracycline antibiotics and determination of impurities in tetracycline by high performance capillary electrophoresis,J. 18(1998): 248-250.

Google Scholar