Determination of Tetracyclines Residues in Aquatic Products by HPLC

Abstract:

A sensitive method for simultaneously determining residues of oxytetracycline, tetracycline ,chlortetracycline and doxycycline in aquatic product was established by HPLC. Samples were extracted with Na₂EDTA-Mcllvaine buffer solutions and centrifuged.The sample extracts were cleaned up by C₁₈ solid-phase extraction cartridge and then were determined by UV detection at 355 nm.At fortification levels of 0.005~0.1 mg/kg, the average recoveries were of 75%~96% , The RSDs were of 1.02%~6.56%,Limit of detection was 0.002 mg /kg for oxytetracycline,tetracycline and doxycycline and 0.005 mg /kg for chlortetracycline.The sensitivity, accuracy and precision of this method were able to meet the requirements for pesticide residue analysis.

• Periodical: Advanced Materials Research (Volumes 1004-1005)
• Pages: 914-918
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1004-1005.914
• Online since: August 2014
• Authors: Ling Lin, Hong Bin Guo, Qi Li, Li Qiang Zhang, Li Li Liu, Chun Liang Yang
• Keywords: Aquatic Products, Chlortetracycline, Doxycycline, HPLC, Oxytetracycline, Tetracycline

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DOI: 10.1365/s10337-003-0068-9

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