Study on Fingerprint of ZheRong Radix Pseudostellariae by HPLC

Abstract:

To establish HPLC fingerprint of ZheRong Radix pseudostellariae and the evidence was provided for quality control and identification of the Chinese crude drug. The HPLC method was used,chromatography conditions were Agilent Eclipse XDB-C18( 4. 6 mm ×250 mm,5 μm) column with gradient mobile phase of acetonitrile-water,The detection wavelength was 203 nm and the col-umn temperature was 30 °C with the flow rate of 1. 0 mL/min,the sample injection was 20μL. HPLC fingerprints of 32 sam-ples of ZheRong Radix pseudostellariae were established. 9 common peaks were selected as the fingerprint peaks in all samples. Among the obtained fingerprints,most of the detected peaks were separated effectively. 32 samples had high similarities. The established HPLC fingerprint has desirable accuracy,repeatability and stability,which can be used for one of the quality control methods of ZheRong Radix pseudostellariae.

• Periodical: Advanced Materials Research (Volumes 1010-1012)
• Pages: 160-163
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1010-1012.160
• Online since: August 2014
• Authors: Ting Xin Wang*, Dong Jun Gong, Li Ya Xia, Xiao Qiang He
• Keywords: Fingerprint, HPLC, Quality Evaluation, ZheRong Radix Pseudostellariae

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