Cloning and Bioinformatic Analysis of Stearoyl-Acyl-Carrier Protein Desaturase Gene in Cassava (Manihot esculenta Crantz)

Jie Fan; Rui Mei Li; Meng Ting Geng; Yuan Yao; Cheng Long Yang; Yu Qing Wang; Rui Jun Duan; Shao Ping Fu; Jiao Liu; Xin Wen Hu; Jian Chun Guo

doi:10.4028/www.scientific.net/AMR.1010-1012.155

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1010-1012Cloning and Bioinformatic Analysis of...

Cloning and Bioinformatic Analysis of Stearoyl-Acyl-Carrier Protein Desaturase Gene in Cassava (Manihot esculenta Crantz)

Abstract:

Stearoyl-acyl-carrier protein desaturase is one of the key enzymes that irreversibly catalyze the biosynthesis of fatty acids, and has an influence on the proportion of saturated and unsaturated fatty acids. In this research, MeSACPD has been cloned from cassava, with 1197 nucleotides and 398 amino acids, respectively. It has high homologous with the stearoyl-acyl-carrier protein desaturase in Ricinus communis. The 3D structure of MeSACPD show a conserve motif related to Fe ion binding. We suppose the motif may be the active center and the substrate binding site area of stearoyl-acyl-carrier protein desaturase.

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Periodical:

Advanced Materials Research (Volumes 1010-1012)

Pages:

155-159

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1010-1012.155

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Online since:

August 2014

Authors:

Jie Fan, Rui Mei Li, Meng Ting Geng, Yuan Yao, Cheng Long Yang, Yu Qing Wang, Rui Jun Duan, Shao Ping Fu, Jiao Liu, Xin Wen Hu, Jian Chun Guo*

Keywords:

Cassava, MeSACPD, Sequence Analysis

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* - Corresponding Author

References

[1] Somerville.C. and Browse.J. Plant lipids: metabolism. mutanits. and membranes. Sciemuce, 1991, 252. 80-87.

Google Scholar

[2] Ohlrogge J.B. Design of new plant products: engineering of fatty acid metabolism. Planizt Plivsiol, 1994, 104. 821-826.

DOI: 10.1104/pp.104.3.821

Google Scholar

[3] RAPOPORT S I, RAO J S, IGARASHI M. Brain metabolism of nutritionally essential polyunsaturated fatty acids depends on both the diet and the liver [J]. Prostaglandins, Leukotrienes and Essential Fatty Acids, 2007, 77: 251-261.

DOI: 10.1016/j.plefa.2007.10.023

Google Scholar

[4] NagaiJ, BloehK. EnzymatiedesaturationofstearylaeylearrierProtein. Jbiol Chem, 1968, 243(17): 4626一4633.

Google Scholar

[5] Strittmatter, P., Spatz, L., Corcoran, D., Rogers, M. J., Set-low, B. & Redline, R. Proc. Natl. Acad. Sci. USA, 1974, 71, 4565-4569.

DOI: 10.1073/pnas.71.11.4565

Google Scholar